Blog Articles2019-02-05T15:45:39-04:00

Blog Articles

Shipping Conditions Q&A

Q: Under what shipping conditions should I send my protein sample? A: We suggest the following shipping conditions: We recommend that you ship your samples in cold conditions, with either multiple cold packs to last travel time or in dry ice. Keep in mind that dry ice will only last 18-24 hours. In the past, we have accepted other options such as lyophilized samples and encourage you to contact us to see what conditions would be most suitable for your sample. Why do we suggest these conditions? Temperature can affect the stability and half-life of proteins. In addition, there [...]

August 19th, 2019|

Chapter 2: Sample Preparation Part I – Purification

General Purification of Proteins, with a focus on antibodies Antibody purity is crucial if the antibody is to be used therapeutically. Monoclonal antibodies (mAbs) are used in the treatment of cancer, infectious diseases, and most recently, migraines [1, 2]. As a result, it is of immense importance that they do not possess contaminants that may lead to an immune reaction and fatal complications. When conducting mass spectrometry to identify, characterize and/or sequence antibodies, it is also important that antibody or other protein samples be free of contaminants. This article highlights common contaminants found in protein or [...]

August 16th, 2019|

Protein Purity Q&A

Q: How much protein and what purity is needed for protein sequencing? A: We typically accept samples at 100 µg with at least 80% purity, rare in the field. To measure the amount of protein, you can use a microvolume spectrophotometer such as the Nanodrop or a traditional Bradford assay. To assess purity of your samples, you may first start by running your sample on a reducing SDS-PAGE acrylamide gel. If you would like to send an antibody, you should be able to see two distinct bands at 25 kDa and 50 kDa for the light and heavy chains, [...]

August 12th, 2019|

Chapter 1: Intro to Protein Sequencing

Welcome to our Mass Spectrometry Proteomics 101 series! Rapid Novor specializes in protein sequencing by mass spectrometry, which is a valuable tool for elucidating the amino acid code of antibodies. However, this technology is still relatively unfamiliar to most researchers. This is why, starting this Friday, we will be posting detailed blogs on proteomics by mass spectrometry. Our goal is to help you become familiar with general proteomics and mass spectrometry definitions that are key to understanding protein sequencing in an accessible and digestible manner. CHAPTER 1: INTRODUCTION Protein sequencing refers to a mass spectrometry-based approach [...]

August 9th, 2019|

Protein Contaminants Q&A

Q: What are common contaminants that could affect my protein sequencing results? A: Typical contaminants that we see affecting samples are non-target proteins, and non-target antibodies [1, 2, 3]. Protein Contaminants The most common protein contaminants include keratin and serum albumin [1, 2, 3]. Other Mass Spectrometry (MS) protein contaminants identified in research labs worldwide by a recent Human Proteome Organization study are casein and E. coli proteins [3]. Keratin is an epidermal structural protein that is found on the outer layer of skin, hair, nails and eventually in dust [1, 3]. Keratin is especially difficult because its size ranges between [...]

August 5th, 2019|

What is Protein Sequencing by Mass Spectrometry?

Frederick Sanger, father of DNA sequencing, sequenced the first protein, insulin, before he began his efforts in deciphering nucleotide codes. Nowadays, DNA sequencing is so popular that it is easy to forget that the first sequenced biological material was protein – insulin, by Sanger [1, 2]. Sanger, and another researcher, Edman, separately pioneered protein sequencing. Edman degradation, also known as Edman sequencing, is still used, though it suffers from many shortcomings in contrast to today’s protein sequencing by Mass Spectrometry (MS). If you would like to learn more about Edman degradation and how it [...]

August 2nd, 2019|

Milestone: 1000 Projects

Meet Anthony: he likes having a quality meal. Balanced. With healthy fats like avocado. Anthony wakes up every morning to the smell of coffee and a strong sense of carpe diem. He doesn’t just employ the art of positive visualization, popularized by The Sopranos. His dogma, if a TV show’s principles could be dogma, is to embrace the spirit of competition and fully seize each day. He leads by example and walks tall, literally. Standing at 6’3”, he gazes ahead with clarity and tackles every challenge. Today, he marked a great milestone for our company, 1000 projects, unprecedented for a [...]

July 29th, 2019|

Highlights from ASMS 2019

This year’s American Society for Mass Spectrometry (ASMS) conference took place in Atlanta, Georgia, the peach state of the US. Our Proteomics Specialist, Kathleen Gorospe, brought back home some delicious peach cookies, for which we have no pictures because they were devoured instantly. The ASMS conference was very fruitful indeed. There was a total of 384 oral presentations, more than 2700 poster presentations, in addition to training courses, and nightly workshops on important topics for the Mass Spectrometry (MS) community. Kathleen (front row, middle) at the Bioinformatics of Protein Identification course at ASMS 2019. Kathleen attended one of [...]

July 17th, 2019|