Glycan Analysis Service via LC-MS.

Discover the locations and relative quantities of common glycans using liquid chromatography and mass spectrometry.

How Glycan Analysis Works

Glycosylation is one of the most common and functionally important post-translational modifications (PTM) that proteins experience. Each protein molecule with the same sequence of amino acids may be glycosylated in a different location or a different way. The most common glycoforms are well understood and are referred to by the number of terminal galactose residues and presence of fucose – (e.g. G2F is a Glycoform with 2 terminal galactose residues, and with a Fucosylated biantennary complex). The relative abundance of G0, G0F, G1, G1F, G2, G2F, etc. in a sample is referred to as the glycan profile. To determine the glycan profile and glycan sites, two experiments are performed in sequence:

  1. To determine the location of the glycosylation sites, we digest the protein resulting in glycopeptides and unmodified peptides. Mapping the modified and unmodified peptides back to the amino acid sequence reveals the glycan sites.
  2. Measure the intact mass of each glycoprotein using liquid chromatography coupled with mass spectrometry to understand their identity and to record the peak area for each glycoform. For antibodies, we can digest with specific enzymes to analyze the Fc and Fab regions.

Applications of our Glycan Analysis service

Glycan analysis can be used to understand a valuable research reagent, a wild type antibody, a recombinantly expressed antibody, or a marketed therapeutic. Glycan information, along with other biophysical characteristics can aid in selection and engineering of antibodies for therapeutics, IVD reagents, controls and research reagents. It can also be used to understand how glycosylation is applied by different expression systems, cell pools, cell lines and production conditions. This informs early decisions in production and is part of quality control and ICH Q6B standards.

The Rapid Advantage.

Identify Glycan Sites.

Fast and routine identification and confirmation of glycan sites informs your selection and protein engineering efforts.

Streamlined Projects.

Reduce cycle time by adding glycan analysis to other services offered by Rapid Novor, including peptide mapping, de novo sequencing, transient expression, SPR and antibody discovery.

Glycan Analysis Pricing.

GLYscan Bulk

$15,000per 10 mAbs
  • Ideal for bulk screening mAbs or cell pools
  • Timeline: 10 days

GLYscan

$2,500
  • Ideal for a single mAb or other protein
  • Timeline: 5 days

GLYscan Intro Offer

$1,500
  • For 1st time clients
  • Timeline: 5 days

Other Glycan Analysis

Inquire
  • Custom projects
  • Timneline: TBD

MATCHmAb™ + GLYscan bundle

$3,000
  • Ideal for cell pool selection, or for pilot production. Volume discounts apply
  • Turn around time: 5 days

REmAb® + GLYscan bundle

$15,999
  • Ideal for understanding sequence and glycan information about a valued reagent
  • Turn around time: 3 weeks

Getting started.

Requirements

  • 100μg of protein
  • >80% purity
  • Protein sequence

Deliverables

  • Glycan site occupancy report
  • Glycan profile (e.g. g0, g0f,  g1, g1f, etc. – % values that sum to 100)
Dominic Narang, PhD
Dominic Narang, PhDManager of HDX and Core Services, Sr. Research Scientist

Talk to Our Scientists.

We Have Sequenced 8000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and developed the first recombinant polyclonal antibody diagnostics.

Talk to Our Scientists.

We Have Sequenced 8000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics

Talk to our scientists. We have sequenced over 8000 antibodies and we are eager to help you.

Other Services.

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REmAb®

Monoclonal antibody sequencing from small antibodysamples, no need for hybridoma or DNA information. Full sequence in record time.
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REpAb®

Polyclonal antibody sequencing and antibody discovery service. Sequence antibodies from blood or a polyclonal mixture.
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MATCHmAb

Antibody verification by real-time peptide mapping. Antibody sequence confirmation service for reproducibility.
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HDX-MS

Mass spec based epitope mapping. Epitope mapping for identify the binding site of an antibody to its corresponding antigen with the highest confidence and resolution.
Explore Epitope Mapping

SPR Analysis

Label-free evaluation of antibody-antigen binding relies on a Surface Plasmon Resonance technology. Combined with our expertise in antibody interactions, this results in high sensitivity and the lowest noise even in complex analytes.
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Quantify the immune response by accurately profiling the vast antibody-protein repertoire and its different isotypes, and subtypes in serum over time.
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