Sequence Variant Analysis Service.
Locate and quantify close variants of your primary sequence within your sample via our Sequence Variant Analysis (SVA) LC-MS service.
How Sequence Variant Analysis by LC-MS Works.
To analyze the amino acid sequence of a monoclonal antibody, we rely on liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The sample is first digested with a protease that cleaves the protein in a predictable pattern. The resulting peptides masses (and their fragments’ masses) are compared with theoretical masses calculated from the expected protein sequence. High quality mass spectrometry data on peptides that do not match the expected mAb sequence are then analyzed. If peptides are found that are close variants – e.g. containing a single amino acid substitution – they can be matched against the intended peptide. The relative quantity of the intended peptide and its variant can be computed by examining the peak areas on the MS1 spectra.
It is also possible to attempt to match some of the mass spectrometry data against a standard or customer provided database of protein sequences to more fully understand the sample.
Why SVA Analysis Matters.
Errors in production are possible – single point mutations can occur, portions of the sequence can be reversed, or deficiencies in the growth media can cause amino acid substitutions. Additional chains can be produced in error. Host cell proteins or other contaminants can be introduced. Identifying these issues early on can help troubleshoot performance problems and prevent future errors in production.
Getting Started.
Requirements
- Amino acid sequence(s) in FASTA format
- 20 μg per analysis per sample*
- >90% purity
- Monoclonal antibody (any isotype, format or fragment)
* detecting extremely rare variants in extremely pure samples may require higher quantities
Deliverables
- List of peptides that closely match expected sequence
- % of variant (by peak area)
- List of spectra that match expected sequence
- Optional: List of peptides that match (standard or customer provided FASTA)
- Optional: List of peptides that do not match
Timeline
Screening
Talk to Our Scientists.
We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.
Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and developed the first recombinant polyclonal antibody diagnostics.
Talk to Our Scientists.
We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.
Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics
Talk to our scientists. We have sequenced over 9000+ antibodies and we are eager to help you.
Other Antibody Characterization Services.
Rapid PTM Analysis
Locate, identify and quantify post-translational modifications. Compare between samples.
Sequence Variant Assessment
Identify and quantify variants of your intended antibody within your sample, and consider other contaminants.
Glycosylation Analysis
Find glycosylation site occupancy, and profile their relative abundance %. Compare many samples.
Peptide Mapping
Maximize coverage and confidence, map, identify and quantify post-translational modifications. Compare between samples.
Disulfide Bond Analyses
Analyse peptides cross linked by disulfide bonds. Quantify disulfide bond shuffling in engineered constructs.
Intact Mass, Custom Analyses
Please inquire about intact mass analysis, subunit analysis, etc.
Other Services.
Antibody Sequencing & Discovery Services.
REmAb®
Monoclonal antibody sequencing from small antibodysamples, no need for hybridoma or DNA information. Full sequence in record time.
Explore REmAb
REpAb®
Polyclonal antibody sequencing and antibody discovery service. Sequence antibodies from blood or a polyclonal mixture.
Explore REpAb
MATCHmAb™
Antibody verification by real-time peptide mapping. Antibody sequence confirmation service for reproducibility.
Explore MATCHmAb
HDX-MS
Mass spec based epitope mapping. Epitope mapping for identify the binding site of an antibody to its corresponding antigen with the highest confidence and resolution.
Explore Epitope Mapping
SPR Analysis
Label-free evaluation of antibody-antigen binding relies on a Surface Plasmon Resonance technology. Combined with our expertise in antibody interactions, this results in high sensitivity and the lowest noise even in complex analytes.
Explore SPR Analysis
NovorIg™
Quantify the immune response by accurately profiling the vast antibody-protein repertoire and its different isotypes, and subtypes in serum over time.
Explore Immune Response Profiling