Peptide Mapping & Analysis2022-10-30T22:27:24-04:00

Reduce Risk and Avoid Delays with Rapid Peptide Mapping Service.

MATCHmAb™ peptide mapping service is a routine, fast and easy solution to verify your antibodies, reduce risk and increase reproducibility at every step of your workflow. Our proprietary MATCHmAb™ technology analyzes and confirms that the sequence of your produced or procured antibody matches the expected sequence, and can identify anomalies for further study.

Benefits of Rapid Peptide Mapping

  • Reduce risk of failure or wasted time – verify early and often to prevent downstream issues
  • Fast answers – answers in days, not weeks
  • Go high-throughput – hundreds of samples per week in our purpose built protein sequencing facility
  • Cost effective – make it part of your routine
  • Easy to use – subscription plan or bulk order make it easy for your team
  • Avoid troubleshooting – a simple first step to avoid re-running work
  • Quality control – check every batch, fast and easy
  • IP Protection – confirm the unique sequence of your antibody

Beyond Binding Assays: Specificity Plus Reproducibility.

Since the protein sequence determines structure and function, rapid confirmation of the protein sequence offers an ideal complement to performance validation assays when revalidating antibodies. Validation is important: recent studies have shown that at least 50% of reagent antibodies cannot recognize their targets specifically, and half or more preclinical studies cannot be reproduced. But validation can be cumbersome, especially as multiple complementary validation experiments should be repeated on each new batch. Furthermore, properties other than binding behavior are often overlooked – causing problems downstream. Our peptide mapping mass spec service offers a precise, repeatable, and full confirmation of the protein sample and sequence information. This ensures the antibody is as expected, reducing the need for revalidation or troubleshooting downstream.

Reproducibility Impacts.

$0
USD $1.7 billion wasted each year on commercial antibodies that don’t work as expected. $25 billion wasted on non-reproducible pre-clinical research.
0%
As many as 50% of reagent antibodies are shown to not recognize their targets. 32% of Hybridomas produce unintended antibodies.
0%
Only 6 out of 53 landmark pre-clinical studies could be reproduced in one study. That’s a staggering 88% failure rate.

Reproducibility impacts.

$0
USD $1.7 billion wasted each year on commercial antibodies that don’t work as expected. $25 billion wasted on non-reproducible pre-clinical research.
0%
As many as 50% of reagent antibodies are shown to not recognize their targets. 32% of Hybridomas produce unintended antibodies.
0%
Only 6 out of 53 landmark pre-clinical studies could be reproduced in one study. That’s a staggering 88% failure rate.

“We got very quick answers that confirmed the sequence of 4 samples and saved us time on troubleshooting.”
– Max London, Research Scientist, Phenomic AI

Who Uses MATCHmAbPeptide Mapping.

Researchers, Antibody Users

Researchers use tool antibodies every day, across many applications. Sequence confirmation is the first step in troubleshooting, or daily quality checks to prevent issues.

Antibody Venders, Producers

Easily verify each new lot. Offer premium Verified by MATCHmAb products, or an add-on service.

Antibody Engineering

Cell line instability could introduce mutations to humanized antibodies. Verify the CDRs have not changed or confirm the full expressed sequence matches the engineered one.

CRO, Custom Developers

Checkpoints to increase confidence. Avoid troubleshooting re-runs with clients.

Who uses MATCHmAb.

Researchers, Antibody Users

Researchers use tool antibodies every day, across many applications. Sequence confirmation is the first step in troubleshooting, or daily quality checks to prevent issues.

Antibody Venders, Producers

Easily verify each new lot. Offer premium Verified by MATCHmAb products, or an add-on service.

Antibody Engineering

Cell line instability could introduce mutations to humanized antibodies. Verify the CDRs have not changed or confirm the full expressed sequence matches the engineered one.

CRO, Custom Developers

Checkpoints to increase confidence. Avoid troubleshooting re-runs with clients.

How It Works: The Evolution of Peptide Mapping & Analysis.

MATCHmAb Traditional Peptide Mapping
Workflow LC-MS/MS, single or dual digest LC-MS only, single enzyme only
Bioinformatics Real-time analysis, purpose built algorithms Post-processing, off the shelf tools
Speed Fast: 1-4 days Slow: weeks
Throughput High: > 100 per week Low
Coverage Higher, and amino acid level Lower, and only peptide level
MATCHmAb
Workflow LC-MS/MS, single or dual digest
Bioinformatics Real-time analysis, purpose built algorithms
Speed Fast: 1-4 days
Throughput High: > 100 per week
Coverage Higher, and amino acid level
 Traditional Peptide Mapping
Workflow LC-MS only, single enzyme only
Bioinformatics Post-processing, off the shelf tools
Speed Slow: weeks
Throughput Low
Coverage Lower, and only peptide level

Pricing.

Single Sample

$1000

Bundles < 384 *

$600/sample **

Monthly Subscription

Ask

* Bundles of 10, 48, 96 and 384 available at a significant discount.
** A setup fee may apply depending on batch size.
Prices are subject to change.

Getting Started is Easy.

Here’s what we need:

  1. 20 μg of each protein of interest
  2. Reference sequence(s) in FASTA
  3. The sample should be reasonably pure and not a polyclonal mixture.

You get:

  1. Confirmation that the protein of interest is in your sample
  2. Coverage down to the amino acid level
  3. Any other observations for further investigation (e.g. suspected mutation, PTM, other dominant forms)

Don’t have the sequence yet?

  • Next Generation Protein Sequencing can derive the sequence directly from the protein sample using REmAb® monoclonal sequencing or REpAb® polyclonal sequencing and discovery.
  • Published sequences are available online. Check the antibody databases.
  • If it’s a commercial antibody, ask your vendor to MATCHmAbTM it
  • If you have access to the hybridoma or cell line, NGS can help.

Learn More.

Moving Towards Biosimilar Drugs

Written by Vanessa Yoon Calvelo, PhD August 3, 2022 Contents What are Biosimilar Drugs? Why are Biosimilars Being Developed? Biosimilars are not [...]

Talk to Our Scientists.

We Have Sequenced 6000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and developed the first recombinant polyclonal antibody diagnostics.

Antibody Sequencing, Discovery and Proteomics Services.

Antibody Sequencing & Discovery Services.

REmAb®

Monoclonal antibody sequencing from small protein samples, no need for hybridoma or DNA information. Full sequence in record time.
Explore REmAb

REpAb®

Polyclonal antibody sequencing and antibody discovery service. Sequence antibodies from blood or a polyclonal mixture.
Explore REpAb

MATCHmAb

Antibody verification by real-time peptide mapping. Antibody sequence confirmation service for reproducibility.
Explore MATCHmAb

HDX-MS

Mass spec based epitope mapping. Epitope mapping for identify the binding site of an antibody to its corresponding antigen with the highest confidence and resolution.
Explore Epitope Mapping

SPR Analysis

Label-free evaluation of antibody-antigen binding relies on a Surface Plasmon Resonance technology. Combined with our expertise in antibody interactions, this results in high sensitivity and the lowest noise even in complex analytes.
Explore SPR Analysis

NovorIg™

Quantify the immune response by accurately profiling the vast antibody-protein repertoire and its different isotypes, and subtypes in serum over time.
Explore Immune Response Profiling

Other Proteomics

We are the go-to proteomics CRO for protein sequencing, performance analysis, discovery, and optimization. Explore the full suite of proteomics services offered by Rapid Novor.
Explore Proteomics Services

We Have Sequenced 4000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics

Talk to our scientists. We have sequenced over 4000 antibodies and we are eager to help you.