Benefits of Rapid Peptide Mapping
- Reduce risk of failure or wasted time – verify early and often to prevent downstream issues
- Fast answers – answers in days, not weeks
- Go high-throughput – hundreds of samples per week in our purpose built protein sequencing facility
- Cost effective – make it part of your routine
- Easy to use – subscription plan or bulk order make it easy for your team
- Avoid troubleshooting – a simple first step to avoid re-running work
- Quality control – check every batch, fast and easy
- IP Protection – confirm the unique sequence of your antibody
Beyond Binding Assays: Specificity Plus Reproducibility.
Since the protein sequence determines structure and function, rapid confirmation of the protein sequence offers an ideal complement to performance validation assays when revalidating antibodies. Validation is important: recent studies have shown that at least 50% of reagent antibodies cannot recognize their targets specifically, and half or more preclinical studies cannot be reproduced. But validation can be cumbersome, especially as multiple complementary validation experiments should be repeated on each new batch. Furthermore, properties other than binding behavior are often overlooked – causing problems downstream. Our peptide mapping mass spec service offers a precise, repeatable, and full confirmation of the protein sample and sequence information. This ensures the antibody is as expected, reducing the need for revalidation or troubleshooting downstream.
Reproducibility Impacts.
Reproducibility impacts.
“We got very quick answers that confirmed the sequence of 4 samples and saved us time on troubleshooting.”
– Max London, Research Scientist, Phenomic AI
Who Uses MATCHmAb™ Peptide Mapping.
Researchers, Antibody Users
Researchers use tool antibodies every day, across many applications. Sequence confirmation is the first step in troubleshooting, or daily quality checks to prevent issues.
Antibody Vendors, Producers
Easily verify each new lot. Offer premium Verified by MATCHmAb™ products, or an add-on service.
Antibody Engineering
Cell line instability could introduce mutations to humanized antibodies. Verify the CDRs have not changed or confirm the full expressed sequence matches the engineered one.
CRO, Custom Developers
Checkpoints to increase confidence. Avoid troubleshooting re-runs with clients.
Who uses MATCHmAb™.
Researchers, Antibody Users
Researchers use tool antibodies every day, across many applications. Sequence confirmation is the first step in troubleshooting, or daily quality checks to prevent issues.
Antibody Venders, Producers
Easily verify each new lot. Offer premium Verified by MATCHmAb™ products, or an add-on service.
Antibody Engineering
Cell line instability could introduce mutations to humanized antibodies. Verify the CDRs have not changed or confirm the full expressed sequence matches the engineered one.
CRO, Custom Developers
Checkpoints to increase confidence. Avoid troubleshooting re-runs with clients.
How It Works: The Evolution of Peptide Mapping & Analysis.
| MATCHmAb™ | Traditional Peptide Mapping | |
|---|---|---|
| Workflow | LC-MS/MS, single or dual digest | LC-MS only, single enzyme only |
| Bioinformatics | Real-time analysis, purpose built algorithms | Post-processing, off the shelf tools |
| Speed | Fast: 1-4 days | Slow: weeks |
| Throughput | High: > 100 per week | Low |
| Coverage | Higher, and amino acid level | Lower, and only peptide level |
| MATCHmAb™ | |
|---|---|
| Workflow | LC-MS/MS, single or dual digest |
| Bioinformatics | Real-time analysis, purpose built algorithms |
| Speed | Fast: 1-4 days |
| Throughput | High: > 100 per week |
| Coverage | Higher, and amino acid level |
| Traditional Peptide Mapping | |
|---|---|
| Workflow | LC-MS only, single enzyme only |
| Bioinformatics | Post-processing, off the shelf tools |
| Speed | Slow: weeks |
| Throughput | Low |
| Coverage | Lower, and only peptide level |
Pricing.
Single Sample
Bundles < 384 *
Monthly Subscription
* Bundles of 10, 48, 96 and 384 available at a significant discount.
** A setup fee may apply depending on batch size.
Prices are subject to change.
Getting Started is Easy.
Here’s what we need:
- 20 μg of each protein of interest
- Reference sequence(s) in FASTA
- The sample should be reasonably pure and not a polyclonal mixture.
You get:
- Confirmation that the protein of interest is in your sample
- Coverage down to the amino acid level
- Any other observations for further investigation (e.g. suspected mutation, PTM, other dominant forms)
Don’t have the sequence yet?
- Next Generation Protein Sequencing can derive the sequence directly from the protein sample using REmAb® monoclonal sequencing or REpAb® polyclonal sequencing and discovery.
- Published sequences are available online. Check the antibody databases.
- If it’s a commercial antibody, ask your vendor to MATCHmAbTM it
- If you have access to the hybridoma or cell line, NGS can help.
Learn More.
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Written by: Vanessa Yoon Calvelo, PhD Updated: January 19, 2023 (Published: June 2, 2022) Contents What are post-translational modifications (PTMs)? Impact [...]
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Written by: Yuning Wang, PhD Updated: January 26, 2023 (Published: June 3, 2022) Contents Introduction The Four Levels of Protein [...]
Talk to Our Scientists.
We Have Sequenced 6000+ Antibodies and We Are Eager to Help You.
Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and developed the first recombinant polyclonal antibody diagnostics.
Antibody Sequencing, Discovery and Proteomics Services.
Antibody Sequencing & Discovery Services.
REmAb®
Monoclonal antibody sequencing from small protein samples, no need for hybridoma or DNA information. Full sequence in record time.
Explore REmAb
REpAb®
Polyclonal antibody sequencing and antibody discovery service. Sequence antibodies from blood or a polyclonal mixture.
Explore REpAb
MATCHmAb™
Antibody verification by real-time peptide mapping. Antibody sequence confirmation service for reproducibility.
Explore MATCHmAb
HDX-MS
Mass spec based epitope mapping. Epitope mapping for identify the binding site of an antibody to its corresponding antigen with the highest confidence and resolution.
Explore Epitope Mapping
SPR Analysis
Label-free evaluation of antibody-antigen binding relies on a Surface Plasmon Resonance technology. Combined with our expertise in antibody interactions, this results in high sensitivity and the lowest noise even in complex analytes.
Explore SPR Analysis
NovorIg™
Quantify the immune response by accurately profiling the vast antibody-protein repertoire and its different isotypes, and subtypes in serum over time.
Explore Immune Response Profiling
Other Proteomics
We are the go-to proteomics CRO for protein sequencing, performance analysis, discovery, and optimization. Explore the full suite of proteomics services offered by Rapid Novor.
Explore Proteomics Services
We Have Sequenced 4000+ Antibodies and We Are Eager to Help You.
Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics