Since the protein sequence determines structure and function, rapid confirmation of the protein sequence offers an ideal complement to performance validation assays when revalidating antibodies. Validation is important: recent studies have shown that at least 50% of reagent antibodies cannot recognize their targets specifically, and half or more preclinical studies cannot be reproduced. But validation can be cumbersome especially as multiple complementary validation experiments should repeated on each new batch. Furthermore, properties other than binding behavior are often overlooked – causing problems downstream. A much more precise, repeatable, and full confirmation of the protein sample ensures the antibody is as expected, reducing the need for revalidation or troubleshooting downstream.