October 2021

Ushering the New Era in Anti-Drug Antibody Assays with Next Generation Protein Sequencing

2021-10-15T13:30:54-04:00October 15th, 2021|Whitepapers|

Anti-drug antibody (ADA) assays are critical to assess the clinical efficacy and safety of a biological drug and rely on control reagents that mimic the ADA response to the biological drug being tested. These positive controls typically consist of animal-derived pooled polyclonal antibodies or human monoclonal antibody reference panels against the target protein drug.

August 2021

What are Monoclonal Antibodies?

2021-10-12T13:04:45-04:00August 25th, 2021|Articles|

Monoclonal antibodies (mAbs) are homogenous antibodies that bind to a single epitope on an antigen. Kohler and Milstein generated the first mAbs when they developed hybridoma technology in the 1970s. Because of the specificity, homogeneity and unlimited availability, mAbs are valuable reagents used in a variety of important applications including treatment and diagnosis of diseases

July 2021

What is the De Novo Amino Acid Sequencing Method?

2021-10-12T13:17:31-04:00July 19th, 2021|Articles|

Proteins are composed of peptide chains, which in turn are made up of a string or linear sequence of amino acids (Figure 1A). Every amino acid has a basic structure containing an amino (-NH2) group and a carboxylic (-COOH) group (Figure 1B). To form a peptide, amino acids link to each other via a peptide bond, which involves the reaction between the carboxylic group of one amino acid and the amine group of another amino acid (Figure 1B). As such, the primary structure of a protein is typically recorded starting at the amino-terminal (N) end and continuing to the carboxyl-terminal (C) end. The primary protein structure may be directly sequenced from a sample of the protein itself or inferred from the DNA sequence.

June 2021

The New Era in ADA Assays

2021-10-05T08:32:34-04:00June 7th, 2021|Webinars|

Anti-Drug Antibody (ADA) assays such as ligand-based assays are critical to assess the clinical efficacy and safety of a biological drug. ADA assays rely on control reagents that mimic the ADA response to the biological drug being tested. These positive controls typically consist of animal-derived (e.g., rabbits) pooled polyclonal antibodies (pAb) [...]

Polyclonal Antibody Sequencing with Only Proteomics

2021-10-05T08:27:24-04:00June 1st, 2021|Webinars|

Landing on the Moon with Mass Spectrometry: Polyclonal Sequencing with Only Proteomics Presented by Anthony Stajduhar, Director of International Business Development, Rapid Novor Over the past 5 years Rapid Novor has perfected monoclonal antibody sequencing, and is now sequencing mAbs from polyclonal mixtures using REpAb®.  After successfully launching their proteogenomics based [...]

March 2021

The Underlying Cause of Medical Diagnostic Invalidation

2021-10-14T12:30:50-04:00March 23rd, 2021|Whitepapers|

In vitro diagnostics (IVDs) are one of the most commonly used tools to diagnose conditions and guide treatment decisions, and are often considered the “silent champion” of healthcare. They work by detecting the absence or presence of particular markers or by measuring the concentration of analytes or specific substances.