October 2022

Selecting Specific Monoclonal Antibodies by SPR – Alpaca Antibody Showdown

2022-11-30T19:36:55-05:00October 30th, 2022|Webinars|

In this SPR webinar, you will learn: How SPR works and its unique advantages for kinetic analysis of antibody-antigen interactions Applications of SPR in antibody discovery Functionality of SPR in antibody characterization Abstract Due to their ability to bind biomolecules specifically and tightly, antibodies are highly [...]

August 2022

What Generates Antibody Diversity?

2022-11-28T11:08:39-05:00August 31st, 2022|Articles|

Written by Genya Gorshtein, MSc August 31, 2022 Contents Introduction Antibody Loci and V(D)J Recombination Somatic Hypermutation Class Switch Recombination De Novo Proteomic Sequencing of Antibodies Introduction Without the presence of an antigen, the human immune system can generate more than 1012 different antibodies and as many [...]

What are Polyclonal Antibodies?

2022-11-17T13:24:32-05:00August 18th, 2022|Articles|

Written by Genya Gorshtein, MSc August 18, 2022 Contents Introduction How are Polyclonal Antibodies Produced? Applications of Polyclonal Antibodies Challenges of Polyclonal Antibodies De Novo Polyclonal Antibody Sequencing Introduction Polyclonal antibodies (pAbs) are a heterogeneous mix of antibodies derived from B cells in the adaptive immune system. [...]

Structure and Function of Antibodies

2022-08-24T17:37:21-04:00August 10th, 2022|Articles|

Written by Genya Gorshtein, MSc August 10, 2022 Contents General Structure of Antibodies Fab and Fc Fragments Hinge Region CDR and FR Regions Antibody Structure Analysis Services General Structure of Antibodies Antibodies or immunoglobulins (Ig) maintain a common quaternary structure consisting of two identical heavy chains (HCs) and two [...]

July 2022

Protein Characterization by HDX-MS

2022-10-27T11:20:06-04:00July 26th, 2022|Articles|

Written by Vanessa Yoon Calvelo, PhD July 21, 2022 Contents Mass spectrometry-based approaches for structural analysis of proteins HDX-MS Applications for ‘Difficult’ Proteins HDX-MS – A powerful analytical technique for protein characterization Mass spectrometry-based approaches for structural analysis of proteins Characterization of proteins and protein complexes is [...]

June 2022

What is Polyclonal Antibody Sequencing?

2022-11-24T16:16:27-05:00June 1st, 2022|Articles|

Written by Yuning Wang, PhD May 31, 2022 Contents Background What is Polyclonal Antibody Sequencing? Applications of Polyclonal Antibody Sequencing Advantages of Polyclonal Antibody Sequencing World’s First: De Novo Polyclonal Antibody Sequencing at Rapid Novor Background The native immune system produces polyclonal antibodies (pAb) by different B-cell [...]

March 2022

February 2022

Camelid Antibodies and Nanobodies

2022-11-17T14:09:21-05:00February 4th, 2022|Articles|

Written by Yuning Wang, PhD January 21, 2022 Contents Discovery of Camelid Antibodies What are Camelid Antibodies? Structure of Camelid Antibodies and Nanobodies Applications of Nanobodies How are Nanobodies Developed? References Discovery of Camelid Antibodies Three decades ago, the discovery1 of a peculiar [...]

January 2022

December 2021

Next Generation Protein Sequencing in Veterinary Medicine and Industry

2022-07-05T14:24:01-04:00December 3rd, 2021|Whitepapers|

Since 2006, the One Health Initiative (OHI)’s goal has been to demonstrate the inextricable link between humans, animals, and the environment. Certainly, the current global pandemic is a great testament to the ties between climate change, humans, and animals that OHI has been working to highlight. The rise of other zoonotic diseases (e.g., Hendra, and Nipah viruses) not only directly affect humans through disease transmission but may also result in deep impacts to the food supply

October 2021

Ushering the New Era in Anti-Drug Antibody Assays with Next Generation Protein Sequencing

2022-04-18T20:15:18-04:00October 15th, 2021|Whitepapers|

Anti-drug antibody (ADA) assays are critical to assess the clinical efficacy and safety of a biological drug and rely on control reagents that mimic the ADA response to the biological drug being tested. These positive controls typically consist of animal-derived pooled polyclonal antibodies or human monoclonal antibody reference panels against the target protein drug.

September 2021

August 2021

De Novo Protein Sequencing vs DNA Sequencing

2022-08-17T18:18:59-04:00August 1st, 2021|Articles|

Written by Yuning Wang, PhD August 1, 2021 What is DNA Sequencing? DNA sequencing is the process of determining the precise order of four nucleotides bases—adenine (A), guanine (G), cytosine (C), and thymine (T)—that make up the DNA molecule. From Sanger sequencing to next-generation sequencing (NGS), DNA sequencing’s accessibility and ease [...]

July 2021

What is Protein Mass Spectrometry?

2022-04-25T15:22:40-04:00July 18th, 2021|Articles|

Protein mass spectrometry refers to the use of mass spectrometry in the study and characterization of proteins, including their quantification, profiling, interaction mapping, and identification of their post-translational modifications (1,2). Protein mass spectrometry may also be referred to as mass spectrometry-based proteomics. Mass spectrometry-based proteomics consist of three approaches: top-down, middle-down, and bottom-up proteomics

How Do You Obtain the Sequence of an Antibody?

2022-11-15T15:14:17-05:00July 10th, 2021|Articles|

Antibody sequences are critical for antibody engineering and protein characterization in therapeutic development. For antibody reagent users, knowing the sequences allows them to perform sequence analysis/alignment to identify binding and cross-reactivity so they can conduct rational experiment design.

June 2016

Moving Towards Recombinant Monoclonal Antibodies

2021-12-14T18:06:16-05:00June 30th, 2016|Articles|

Recombinant Monoclonal Antibodies (rAbs) are highly reproducible, customizable and pure alternatives to the traditional antibodies produced by hybridomas. Get the antibody protein sequence, either by DNA sequencing or the de novo protein sequencing technology, you can rest assured that you can have the exact antibody made recombinantly anytime in the future.