Polyclonal Antibody Sequencing2024-05-14T15:10:56-04:00

Polyclonal Antibody Sequencing.

Use REpAb® polyclonal antibody sequencing to convert your pAb to a set of mAbs. Get the robust performance of your polyclonal antibody and the reliability of recombinant antibodies, by sequencing the antibodies within the pAb and recombinantly expressing the dominant form(s).

How Polyclonal Antibody Sequencing Works.

Through proprietary chemistry, separation, sample preparation and artificial intelligence, dozens of mass spectrometry experiments come together to derive the full sequences of mAbs within a polyclonal mixture. The workflow involves multiple enzymes and uses bottom-up, top-down and middle-down mass spectrometry. AI-powered de novo sequencing and assembly algorithms are used to overlay the enormous data set to determine the full antibody sequences.

monoclonal antibody sequencing workflow through de novo protein sequencing
REmAb
Highest throughput: 50 to 100 mAbs per week
Highest accuracy: 100%
Lowest sample requirement: 50 μg
Most enzymes: 5-10 digests
Proprietary, next generation algorithms
Ile/Leu calls with WILD and WILDtag technology
Ability to handle the unexpected
Highest coverage: 100X
One sequence that works, every time

Unique Technology for pAb Sequencing.

REpAb is the only commercial polyclonal antibody de novo protein sequencing platform – we are uniquely capable of sequencing antibodies from a complex polyclonal mixture without reference to a sequence database or genomic information.

Applications of Polyclonal Antibody Sequencing.

As a stand-alone service, polyclonal antibody sequencing is most commonly used in development of in-vitro diagnostics (IVD). IVD that are based on pAbs are highly dependent on the producing animal, and are subject to costly revalidation and batch-to-batch variability. Converting them to rmAbs leads reduces supply chain risk, and eliminates revalidation concerns.

Antibody Sequencing Success Stories.

“You are certainly the best CRO we’ve ever worked with!❤️”

Roya Servattalab, Scientist II, Marengo Therapeutics

“Rapid Novor was tasked with finding highly specific, high affinity antibodies against an important target – and they delivered powerful antibodies fit-for-purpose. Such antibodies are not easily obtained yet Rapid Novor approach proved immediately successful, despite our time-constrained and stringent demands”

Matt Pope, Principal Scientist, SISCAPA
0%
Of client projects have returned mAb binders
0
Polyclonal sequencing projects, since 2020.
0
Average number of pAb sequences returned from a pAb. Range is 2-85.

Getting Started With Polyclonal Antibody Sequencing.

Requirements

  • 1 mg polyclonal antibody – must be antigen-affinity and protein A/G purified IgG
  • Most species (rabbit, chicken, goat, sheep, camelid, dog, etc.)
  • Single host (not pooled); pooling from multiple bleeds of a single host is acceptable
  • Optional: 100 μg antigen protein
  • Optional: NGS data or PBMCs from the same host at a similar time point

Deliverables

  • Full length heavy and light chains
  • Chain pairing, including any alternate pairs observed
  • Ranking (based on confidence/abundance)
  • Coverage maps

Monoclonal or Oligoclonal Antibody Sequencing

REmAb® is our other sequencing service, designed for the simpler problem of sequencing monoclonal proteins. It can also handle multiple chains or oligoclonal mixtures of a handful of antibodies. Learn about REmAb Antibody Sequencing Service.

Antibody Discovery

REpAb® polyclonal sequencing technology can be used in an antibody discovery context, starting from antigen synthesis or immunization, and carrying through to pre-clinical development. Please see our Antibody Discovery Service page for details.

Antibody Sequencing Pricing.

The price for a REpAb project depends on the nature of the starting material (number and quality of pAbs to sequence), and the objectives. Please inquire for details.

Optional Add Ons.

Easy Purchasing Options.

Talk to Our Scientists.

We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and developed the first recombinant polyclonal antibody diagnostics.

Talk to Our Scientists.

We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics

Talk to our scientists. We have sequenced over 9000+ antibodies and we are eager to help you.

REpAb® De Novo Polyclonal Antibody Sequencing Applications.

Other Antibody Sequencing, Discovery and Characterization Services.

Antibody Sequencing & Discovery Services.

REmAb® mAb Sequencing

Monoclonal antibody sequencing from small antibodysamples, no need for hybridoma or DNA information. Full sequence in record time.
Explore Antibody Sequencing Services

REmAb® Protein Sequencing

Monoclonal antibody sequencing from small antibodysamples, no need for hybridoma or DNA information. Full sequence in record time.
Explore Antibody Sequencing Services

MATCHmAb

Antibody verification by rapid peptide mapping. Antibody sequence confirmation and full detailed mapping for development purposes.
Explore Peptide Mapping

RapidHDX-MS

Mass spec based epitope mapping. Epitope mapping for identify the binding site of an antibody to its corresponding antigen with the highest confidence and resolution.
Explore HDX-MS Epitope Mapping

RapidSPR Analysis

Label-free evaluation of antibody-antigen binding using surface plasmon resonance on Biacore or Nicoya. Can be employed for detailed kinetic profiling, kinetics screening, or epitope binning.
Explore SPR Analysis

Antibody Characterization

LC-MS analysis for study of antibody glycosylation, disulfide bonds, post-translational modifications, sequence variants, intact mass and size.
Explore antibody characterization