Hybridoma Loss Prevention2022-08-17T20:00:34-04:00

Hybridoma Loss Insurance.

Hybridoma instability is common and costly.

Although significant amounts of time and effort are spent on culturing hybridoma cell lines, poor growth or even cell death still occurs occasionally. Many factors, including long-term storage, repeated freeze-thaw cycles, improper handling, and contamination can all lead to failure of hybridoma survival causing permanent loss of important mAbs. Hybridoma cell lines are also known to undergo gene mutations and rearrangements over time, leading to antibody heterogeneity and batch-to-batch variability – ie. hybridomas can contain additional heavy or light chain genes resulting in impaired affinity and specificity to the target antigen; hybridomas also may lose the chromosome containing the antibody genes required for antibody generation.

Next generation protein sequencing (NGPS) can provide the amino acid sequence of any antibody to help recover from the loss, death or mutation of hybridoma cell lines. Indefinite access to the amino acid sequence can mean a limitless supply of commonly used antibodies through recombinant expression, providing an insurance policy in case they are discontinued. NGPS can also validate every batch of antibodies produced by hybridomas, confirming batch-to-batch consistency.

Webinar

Rational Antibody Design and Engineering with Next Generation Protein Sequencing

Oct 20, 11 am EST

Services for Hybridoma Users.

We support all hybridoma users, reagent and kit manufacturers, and the drug development pipeline by securing and validating the protein sequence. Contact us to discover how we can help provide insurance for your valuable immunoreagents.

Antibody Sequencing & Discovery Services.

de novo mAb Sequencing

Starting with only a small protein sample, resurrect or secure the mAb by obtaining the amino acid sequence even after the death or mutation of a hybridoma cell line.
Explore REmAb

Quality Control and Troubleshooting

Save time troubleshooting cell line stability issues throughout your pipeline, and routinely confirm that the sequences of manufactured immunoreagents match the expected sequences.
Explore MATCHmAb

Recombinant Expression

Secure the protein sequence and safeguard a limitless mAb supply through recombinant expression, and for IP applications, in a streamlined workflow.
Explore Antibody Expression

Application Publications.

Why is Protein Sequencing Useful?

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Protein sequencing is a method that typically utilizes mass spectrometry (MS) to determine the amino acid code of a protein1. Prior to the development of mass spectrometry, Edman degradation, a method involving the stepwise degradation of peptides to derive the order of amino acids, was the mainstream approach. Nowadays, mass spectrometry is favored due to its ease of use and high throughput capabilities, though Edman degradation is still employed for specific applications in which the technique is well suited.

Antibody Validation and its Use Cases

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Antibodies are used in a variety of ways in academia and industry, from tools to therapeutics. Because antibodies are produced using live processes, which are naturally error-prone, validation is required from time to time. Furthermore, to develop biological therapeutics, the protein sequence must be confirmed as part of the regulatory process.

DNA Sequencing vs Next Generation Protein Sequencing

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The protein sequence is key to understanding the function of a protein target and is critical to therapeutic and diagnostic development. This is particularly important for antibodies whose code diversity and glycosylation impact both function, and stability.

“The sequences have allowed us to have an “insurance policy” of sorts for critical proteins used in our various products.”

David Ankrapp, Senior Research Scientist, NEOGEN

Talk to Our Scientists.

We Have Sequenced 6000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and developed the first recombinant polyclonal antibody diagnostics.

Talk to Our Scientists.

We Have Sequenced 6000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics

Talk to our scientists. We have sequenced over 6000 antibodies and we are eager to help you.