August 22, 2024 | 11:00AM EST | 3:00PM UTC In this webinar, you will: Explore how using a proteomics approach with REpAb® de novo sequencing accesses a larger diversity of antibodies, and enhances antibody discovery workflows. Case study on de novo sequencing functional and neutralizing antibodies directly from the serum of a Human [...]
Written by: Genya Gorshtein, MSc Published: June 10, 2024 Contents Introduction Alanine Scanning Mutagenesis Epitope Mapping HDX-MS Epitope Mapping Antibody Epitope Mapping Services Introduction Epitope mapping elucidates the antibody function by identifying specific binding sites on the antigen that interact with the antibody. Alanine [...]
Written by: Genya Gorshtein, MSc Published: May 29, 2024 Contents Introduction What is Epitope Binning Value of Epitope Binning in Early Discovery Stages Epitope Binning with SPR on Biacore 1K Introduction An epitope is an inherent property of an antibody that cannot be altered [...]
Written by: Genya Gorshtein, MSc Published: April 19, 2024 Contents Introduction Bispecifics and Multispecific Antibody Formats Mechanism of Action of Bispecific Antibodies Bispecific and Multispecific Development with Rapid Novor Introduction The complex nature of diseases often limits the therapeutic efficacy of monovalent antibodies. To [...]
Written by: Genya Gorshtein, MSc Published: April 3, 2024 Contents Introduction Membrane Proteins are Difficult Antigens Strategies for Utilizing Membrane Proteins as Antigens in Antibody Discovery In Vivo Antibody Discovery Using Mass Spectrometry Introduction Membrane proteins orchestrate a wide range of cellular functions, such [...]
Written by: Genya Gorshtein, MSc Published: September 14, 2022 Contents Introduction ADCs as Novel Anti-Cancer Chemotherapeutics Key Components of ADCs Future Generation of ADCs De Novo Protein Sequencing Applications in ADC Development Introduction An antibody-drug conjugate (ADC) is a monoclonal antibody (mAb) with a covalently attached [...]
Written by: Genya Gorshtein, MSc Published: August 18, 2022 Contents Introduction How are Polyclonal Antibodies Produced? Applications of Polyclonal Antibodies Challenges of Polyclonal Antibodies De Novo Polyclonal Antibody Sequencing Introduction Polyclonal antibodies (pAbs) are a heterogeneous mix of antibodies derived from B cells in the [...]
Written by Genya Gorshtein, MSc August 10, 2022 Contents General Structure of Antibodies Fab and Fc Fragments Hinge Region CDR and FR Regions Antibody Structure Analysis Services General Structure of Antibodies Antibodies or immunoglobulins (Ig) maintain a common quaternary structure consisting of two identical heavy chains (HCs) and [...]