Epitope Mapping Service via HDX-MS.

Mass spectrometry based epitope mapping service, that can identify the binding site of an antibody to its corresponding antigen with the highest confidence and resolution – understand complex binding, identify linear and conformational epitopes

  • Lower sample requirement – only 200μg required
  • Higher resolution – 1 to 5 amino acids
  • Faster turn around time – clean data in 4 weeks
  • Industry leading coverage

HDX-MS Epitope Mapping Overview & Applications

HDX-MS Overview

Hydrogen-Deuterium-eXchange Mass Spectrometry (HDX-MS) measures the exposure levels of amide hydrogen atoms on the surface of a protein or protein complex. The first step causes the exposed hydrogen atoms to be readily exchanged with deuterium. Hydrogen atoms that are buried within the structure – for instance at the binding site of an antibody-antigen complex – are not exchanged as readily. By examining the peptides using Bottom-up mass spectrometry, and comparing them with the amino acid sequence, it is then possible to determine which regions are the binding locations between the interacting partners.

hdx ms epitope mapping applications

Antibody-Antigen Epitope Mapping Studies

Augmenting Epitope Binning Studies (e.g. via SPR)

Biosimilar Characterization and Comparability

Guided Antibody Discovery

Batch-to-batch Reproducibility for Antibody Production

Antibody Engineering and Optimization

HDX-MS Epitope Mapping Workflow

Workflow

  1. Labeling – incubate different fractions with D2O solution in a time series
  2. Quench – chill and control temperature to prevent further exchange or back-exchange
  3. Digest – prepare peptides for analysis
  4. LC-MS/MS
  5. Data processing – determine deuterium uptake of digested peptides acid and identify binding site(s)
  6. Comparison – compare similar samples – e.g. antibodies with similar sequences
hdx ms workflow

Requirements

  1. 20 μM of each protein solution – e.g. for antibodies 200μg of antigen and the antibody for each epitope. Any species any isotype
  2. Protein sequences (we can perform sequencing if required)
  3. Tell us what you hope to achieve
  4. We’ll provide a timely quote


*Ask us
. We sequenced many mAbs when samples were much less pure than our requirements.

Deliverables

  • Report of regions showing relative deuterium uptake on the sequence and on a 3D structure
  • Indication of the epitope region
Dominic Narang, PhD
Dominic Narang, PhDResearch Scientist

“With 6000+ antibody proteomics projects completed and 6 years of experience in HDX-MS we are able to achieve higher coverage with more overlapping peptides, and resolve HDX regions to within a few amino acids using our multi-enzyme approach. We have helped hundreds of scientists around the globe understand their antibody binding by providing clean data for their research. Let’s chat.

High Resolution Even for Large Ab:Ag Complexes.

We are able to achieve higher coverage with more overlapping peptides, and resolve HDX regions to within a few amino acids using our multi-enzyme approach

Quality and Expertise in Epitope Mapping.

Quality and expertise with over 6 years of experience in HDX-MS capable of working with a variety of analytes and ligands, and novel projects

Complete Epitope Characterization and Antibody Sequencing Platform.

Complete antibody development platform starting from antibody isolation to sequencing. With 6000+ sequencing projects and 6+ years of experience in epitope mapping using mass spectrometry

HDX-MS Epitope Mapping Pricing.

HDX-MS Epitope Mapping

$25,000+
  • Detailed epitope mapping analysis for 1 antibody-antigen pair.
  • Sample requirements: Antigen: approx 200 μg required Ab: 200 μg required
  • Turn around time 4 weeks (Faster if expedited)

HDX-MS Epitope Screening

$10,000+
  • Medium-throughput analysis of binding location of 10+ antibodies to one antigen.
  • Sample requirements: Antigen Approx 500 μg Antibodies 100 μg required of each antibody
  • Turn around time 4 weeks (Faster if expedited)

Custom HDX-MS Project

$25,000+
  • Custom analysis of protein complexes, conformation changes, ligands and other structural questions.
  • Please inquire about experiment design, and sample requirements.
  • .

“The sequences have allowed us to have an “insurance policy” of sorts for critical proteins used in our various products.”

David Ankrapp, Senior Research Scientist, Neogen Corporations

“We’re focused on coupling cytokines with a highly engineered VHVL of an antibody. So by sequencing the antibodies with Rapid Novor we are able to pull out the CDR to graft them into our platform and test in vitro and in vivo.”

Pavel Khrimian – Co-founder and CBO, Deka Biosciences

Talk to Our Scientists.

We Have Sequenced 7000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and developed the first recombinant polyclonal antibody diagnostics.

Talk to Our Scientists.

We Have Sequenced 6000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics

Talk to our scientists. We have sequenced over 6000 antibodies and we are eager to help you.

Antibody Sequencing, Discovery and Proteomics Services.

Antibody Sequencing & Discovery Services.

REmAb®

Monoclonal antibody sequencing from small antibodysamples, no need for hybridoma or DNA information. Full sequence in record time.
Explore REmAb

REpAb®

Polyclonal antibody sequencing and antibody discovery service. Sequence antibodies from blood or a polyclonal mixture.
Explore REpAb

MATCHmAb

Antibody verification by real-time peptide mapping. Antibody sequence confirmation service for reproducibility.
Explore MATCHmAb

HDX-MS

Mass spec based epitope mapping. Epitope mapping for identify the binding site of an antibody to its corresponding antigen with the highest confidence and resolution.
Explore Epitope Mapping

SPR Analysis

Label-free evaluation of antibody-antigen binding relies on a Surface Plasmon Resonance technology. Combined with our expertise in antibody interactions, this results in high sensitivity and the lowest noise even in complex analytes.
Explore SPR Analysis

NovorIg™

Quantify the immune response by accurately profiling the vast antibody-protein repertoire and its different isotypes, and subtypes in serum over time.
Explore Immune Response Profiling