Epitope Mapping Services via HDX-MS2023-10-31T21:59:55-04:00

Epitope Mapping Service via HDX-MS.

Mass spectrometry based epitope mapping service, that can identify the binding site of an antibody to its corresponding antigen with the highest confidence and resolution. Our HDX-MS service can study complex binding, identify linear and conformational epitopes.

How HDX-MS Epitope Mapping Works

HDX-MS Epitope Mapping Workflow

Hydrogen-Deuterium-eXchange Mass Spectrometry (HDX-MS) measures the exposure levels of amide hydrogen atoms on the surface of a protein or protein complex. The first step causes the exposed hydrogen atoms to be readily exchanged with deuterium. Hydrogen atoms that are buried within the protein structure – for instance at the binding site of an antibody-antigen complex – are not exchanged as readily. By examining the peptides using Bottom-up mass spectrometry, and comparing them with the amino acid sequence, it is then possible to determine which regions are the binding locations between the interacting partners.

  1. Labeling – incubate different fractions with D2O solution in a time series
  2. Quench – chill and control temperature to prevent further exchange or back-exchange
  3. Digest – prepare peptides for analysis
  4. LC-MS/MS
  5. Data processing – determine deuterium uptake of digested peptides acid and identify binding site(s)
  6. Comparison – compare similar samples – e.g. antibodies with similar sequences
hdx ms workflow

Applications of our HDX-MS Service

While our HDX-MS service is commonly used for epitope mapping in an antibody-antigen interaction, it can also be used to study other protein-protein interactions, protein conformation changes, multi-protein complexes, small molecule interactions with proteins, disulfide linked proteins, glycosylation sites, protein interactions with DNA or RNA, disordered proteins, etc.

hdx ms epitope mapping applications

The Rapid HDX Advantage.

High Resolution Even for Large Ab:Ag Complexes.

We are able to achieve higher coverage with more overlapping peptides, and resolve epitope regions to within a few amino acids using our multi-enzyme approach.

Quality and Expertise in Epitope Mapping.

With over 7 years of experience in HDX-MS, we are capable of working with a variety of analytes and ligands, and novel projects. No two projects are ever the same and so experimental conditions are always individually optimized for overall project success.

Part of a Complete Epitope Characterization and Antibody Sequencing Platform.

Our HDX-MS service is one part of our antibody solution platform that also offers isolation, protein sequencing, recombinant expression, kinetic analysis and epitope mapping. Combining more than one service into a single workflow gives convenient access to richer, higher quality results.

HDX-MS Epitope Mapping Pricing.

HDX-MS Epitope Mapping

$25,000+
  • Detailed epitope mapping analysis for 1 antibody-antigen pair.
  • Sample requirements: Antigen 200 μg, Antibody 200 μg
  • Turn around time: 4 weeks

HDX-MS Epitope Screening

$10,000+
  • Fast analysis of binding location of 10+ antibodies to one antigen.
  • Sample requirements: Antigen 500 μg, Antibodies 100 μg each
  • Turn around time: 4 weeks

Custom HDX-MS Project

$19,500+
  • Custom analysis of protein complexes, conformation changes, ligands and other structural questions.
  • Please inquire about experiment design, and sample requirements.

Getting started.

Requirements

  1. For antibodies 200μg of antigen and the monoclonal antibody for each epitope.
  2. For other proteins 20 μM of each
  3. Protein sequences (we can perform sequencing if required)

Deliverables

  • Report of regions showing relative deuterium uptake on the sequence and on a 3D protein structure
  • Indication of the epitope region(s)
  • Uptake plots and heatmap
Dominic Narang, PhD
Dominic Narang, PhDManager of HDX and Core Services, Sr. Research Scientist

Talk to Our Scientists.

We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and developed the first recombinant polyclonal antibody diagnostics.

Talk to Our Scientists.

We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics

Talk to our scientists. We have sequenced over 9000+ antibodies and we are eager to help you.

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