Antibody-Antigen Interaction & Binding Kinetics Analysis via SPR.
Surface Plasmon Resonance services can determine the binding affinity, on-rate and off-rate (Kon, Koff and KD) for antibody-antigen interaction. Full kinetic profiling using our expertise in SPR for antibodies.
- Unmatched accuracy and reproducibility
- Responsive – no wait time, answers in 2 weeks
- Low sample volume requirement
- Full kinetic profile from antibody experts
SPR Analysis Workflow
How It Works.
Our method for label free evaluation of antibody-antigen binding relies on a Surface Plasmon Resonance technology from Nicoya called OpenSPR. This nanotechnology-based SPR platform enables immobilization of binding partners in both orientations with low levels for bulk shift even in most complex buffers. Combined with our expertise in antibody interactions, this results in high sensitivity and the lowest noise even in complex analytes.
Compared to ELISA, BLI and label-based approaches for examining binding, SPR has advantages:
- Highest quality measurement of binding kinetics, under realistic conditions
- Flow through, so re-binding events not an issue
- Label free (so not limited by labelling efficiency, specificity, etc.)
- Small sample volume
- High sensitivity
- Can be high throughput
- Real time: ideal for measuring fast on and off rates, deeper insights
- Highly replicable and reproducible
Requirements
- Antibody (100 μg, >90% purity, monoclonal)
- Antigen (at least 100 μg, >90% purity)
- Any standard buffer. Inquire about serum analysis.
*Ask us. If your samples don’t meet our requirements, please get in touch. We may be able to work with them.
Turn-Around Time
Single Ag-Ab pair: 2 weeks
Rush: 1 week*
Binning/screening: inquire
Rapid respond to calls/emails. Projects start on arrival – no queue.
* sample dependent
“If you’re looking for the most accurate SPR results, you need to optimize the binding conditions, the immobilization conditions and the regeneration conditions for each interaction. Fully automated systems and report generation will miss things.”
Unmatched SPR Accuracy and Reproducibility.
Kinetic analysis and validation is performed by our PhD experts in triplicate to meet the highest accuracy standards in the industry. We analyze residuals for validation of fit curve accuracy, and conduct advanced biophysical analysis. Kinetic parameters are reported with standard deviation of the triplicate set (and not just a standard error of a single run).
Expert Analysis and Deeper Insights.
Optimization of binding conditions for every interaction are performed individually for increased accuracy and optimal signal/noise ratio. We provide deeper insights into each interaction with our full regeneration screen using 8 different conditions (acidic, basic, chaotropic and high salt).
Complex SPR and Difficult Samples.
We work with antibodies and antibody fragments, fab, scvf, mAbs or pAbs, peptide antigens and large complex protein antigens, even protein complexes. With our expertise we can work with novel projects that don’t fit standard workflows.
Applications of SPR Analysis
While there are many possible applications of SPR Rapid Novor is highly focused on antibody research and development:
Affinity measurements
Off-rate ranking analysis
Cross-reactivity and specificity studies
Therapeutic antibody developability
Peptide library screening
Custom assay development
SPR Services Pricing.
List Price
- Additional Ligand-Analyte Analysis – $3000
- Bulk Order Discounts Available
- Ligand-Chip Coupling Optimization
- Regeneration Optimization
- Acquiring KD, Kon, Koff
*Per sample fees, setup and optimization costs depend on project requirements. Please inquire.
“The sequences have allowed us to have an “insurance policy” of sorts for critical proteins used in our various products.”
“We’re focused on coupling cytokines with a highly engineered VHVL of an antibody. So by sequencing the antibodies with Rapid Novor we are able to pull out the CDR to graft them into our platform and test in vitro and in vivo.”
Talk to Our Scientists.
We Have Sequenced 7000+ Antibodies and We Are Eager to Help You.
Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and developed the first recombinant polyclonal antibody diagnostics.
Talk to Our Scientists.
We Have Sequenced 6000+ Antibodies and We Are Eager to Help You.
Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics
Talk to our scientists. We have sequenced over 6000 antibodies and we are eager to help you.
Antibody Sequencing, Discovery and Proteomics Services.
Antibody Sequencing & Discovery Services.
REmAb®
Monoclonal antibody sequencing from small antibodysamples, no need for hybridoma or DNA information. Full sequence in record time.
Explore REmAb
REpAb®
Polyclonal antibody sequencing and antibody discovery service. Sequence antibodies from blood or a polyclonal mixture.
Explore REpAb
MATCHmAb™
Antibody verification by real-time peptide mapping. Antibody sequence confirmation service for reproducibility.
Explore MATCHmAb
HDX-MS
Mass spec based epitope mapping. Epitope mapping for identify the binding site of an antibody to its corresponding antigen with the highest confidence and resolution.
Explore Epitope Mapping
SPR Analysis
Label-free evaluation of antibody-antigen binding relies on a Surface Plasmon Resonance technology. Combined with our expertise in antibody interactions, this results in high sensitivity and the lowest noise even in complex analytes.
Explore SPR Analysis
NovorIg™
Quantify the immune response by accurately profiling the vast antibody-protein repertoire and its different isotypes, and subtypes in serum over time.
Explore Immune Response Profiling