Antibody-Antigen Interaction & Binding Kinetics Analysis via SPR.

Our Surface Plasmon Resonance (SPR) service can determine the on-rate (Kon), off-rate (Koff), dissociation constant (Kd), and affinity (KD) for antibody-antigen interaction. Full kinetic profiling using our expertise in SPR analysis for antibodies.

How SPR Works.

surface plasmon resonance workflow for spr based kinetics analysis

How It Works.

Our method for label free evaluation of antibody-antigen binding and biomolecular interaction relies on a Surface Plasmon Resonance analysis technology from Nicoya called OpenSPR. This nanotechnology-based SPR platform enables immobilization of binding partners in both orientations on the sensor chip with low levels for bulk shift even in most complex buffers. Combined with our expertise in antibody interactions, this results in high sensitivity and the lowest noise even in complex analytes.

SPR BLI MST ELISA
Label-free
High-sensitivity (low pM)
Real time, continuous flow
Full Kinetic Analysis
Highest precision
Highest reproducibility

The Rapid SPR Advantage.

Unmatched SPR Accuracy and Reproducibility.

Kinetic analysis and validation is performed by our PhD experts in triplicate to meet the highest accuracy standards in the industry. We analyze residuals for validation of fit curve accuracy, and conduct advanced biophysical analysis. Kinetic parameters are reported with standard deviation of the triplicate set (and not just a standard error of a single run).

Expert Analysis and Deeper Insights.

Optimization of binding conditions for every interaction are performed individually for increased accuracy and optimal signal/noise ratio. We provide deeper insights into each interaction with our full regeneration screen using different conditions (acidic, basic, chaotropic and high salt).

Complex SPR and Difficult Samples.

We work with antibodies and antibody fragments, fab, scvf, mAbs or pAbs, peptide antigens and large complex protein antigens, even protein complexes. With our expertise we can work with novel projects that don’t fit standard workflows.

Applications of SPR Analysis

While there are many possible SPR based molecular interactions studies, Rapid Novor is highly focused on antibody research and development:

Affinity measurements

Off-rate ranking analysis

Cross-reactivity and specificity studies

Drug Discovery, antibody developability

Peptide library screening

Custom assay development

Success Stories.

“The sequences have allowed us to have an “insurance policy” of sorts for critical proteins used in our various products.”

David Ankrapp, Senior Research Scientist, Neogen Corporations

“We’re focused on coupling cytokines with a highly engineered VHVL of an antibody. So by sequencing the antibodies with Rapid Novor we are able to pull out the CDR to graft them into our platform and test in vitro and in vivo.”

Pavel Khrimian – Co-founder and CBO, Deka Biosciences

Getting Started.

Requirements

  • Antibody (100 μg, >90% purity, monoclonal)
  • Antigen (at least 100 μg, >90% purity)
  • Any standard buffer. Inquire about serum analysis.

*Ask us. If your samples don’t meet our requirements, please get in touch. We may be able to work with them.

Deliverables

Full kinetic profile:

  • Kon, Koff, Kd
  • Isoaffinity analysis
  • Optimization and regeneration conditions

High throughput screening:

  • Ranking based on kinetic parameters

Turn-Around Time

Rapid response to calls/emails. Projects start on arrival – no queue.

Kinetic Profile – 3 weeks
mAb Screening – 3 weeks
Rush order – 2 weeks

Timing may be sample dependent.

SPR Services Pricing.

Custom Kinetic Profile

$6,000First Pair
  • $3000 per additional binding pair
  • Suitable for detailed analysis of a few interactions
  • Optimized conditions per analysis
  • Triplicate experiments
  • Highly accurate Kon, Koff and KD profile
  • Isoaffinity analysis

Ab-Ag Kinetic Profile

$3,900First Pair
  • $1950 per additional binding pair
  • Suitable for detailed analysis of a few antibodies
  • Optimized conditions per analysis
  • Triplicate experiments
  • First time customer only
  • Highly accurate Kon, Koff and KD profile
  • Isoaffinity analysis

Antibody Screening by SPR

$950Per Antibody
  • Minimum 10 antibodies
  • Suitable for selecting the best antibodies from a larger set
  • Standard conditions
  • Single experiment
  • Binding affinity and off rate screen
  • Lead ranking

*Per sample fees, setup and optimization costs depend on project requirements. Please inquire.

Talk to Our Scientists.

We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and developed the first recombinant polyclonal antibody diagnostics.

Talk to Our Scientists.

We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics

Talk to our scientists. We have sequenced over 9000+ antibodies and we are eager to help you.

Antibody Sequencing, Discovery and Proteomics Services.

Antibody Sequencing & Discovery Services.

REmAb®

Monoclonal antibody sequencing from small antibodysamples, no need for hybridoma or DNA information. Full sequence in record time.
Explore REmAb

REpAb®

Polyclonal antibody sequencing and antibody discovery service. Sequence antibodies from blood or a polyclonal mixture.
Explore REpAb

MATCHmAb

Antibody verification by real-time peptide mapping. Antibody sequence confirmation service for reproducibility.
Explore MATCHmAb

HDX-MS

Mass spec based epitope mapping. Epitope mapping for identify the binding site of an antibody to its corresponding antigen with the highest confidence and resolution.
Explore Epitope Mapping

SPR Analysis

Label-free evaluation of antibody-antigen binding relies on a Surface Plasmon Resonance technology. Combined with our expertise in antibody interactions, this results in high sensitivity and the lowest noise even in complex analytes.
Explore SPR Analysis

NovorIg™

Quantify the immune response by accurately profiling the vast antibody-protein repertoire and its different isotypes, and subtypes in serum over time.
Explore Immune Response Profiling