Surface Plasmon Resonance Service.

RapidSPR™ is our Surface Plasmon Resonance (SPR) service, which determines the on-rate (kon), off-rate (koff), and antibody affinity (KD) for antibody-antigen interactions. We offer detailed antibody kinetic profiling and SPR assay development, antibody screening SPR and epitope binning SPR. Our expertise is Antibody-Antigen Interaction & Binding Kinetics Analysis.

How SPR Analysis Works.

surface plasmon resonance workflow for spr based kinetics analysis

Our method for label free evaluation of antibody binding and biomolecular interaction relies on a Surface Plasmon Resonance analysis technology from Biacore and Nicoya. This nanotechnology-based SPR platform enables immobilization of binding partners in both orientations on the sensor chip with low levels of bulk shift, high sensitivity and low noise. Our Biacore platform allows for higher throughput SPR services with excellent results.

SPR BLI MST ELISA
Label-free
High-sensitivity (low pM)
Real time, continuous flow
Full Kinetic Analysis
Highest precision
Highest reproducibility

The Rapid SPR Service Advantage.

Reliability, Reproducibility

Highest standards in the industry, using state of the art SPR Biacore 1K systems.

Fast turn-around time

Kinetics analysis complete in 2 weeks or less, with no wait time.

Low sample requirement

Highly sensitive, so we use less of your precious sample. 100 μg or less.

Tolerance for sample purity

Nanotech SPR: low levels of bulk shift even in complex analytes.

Communication and service

Fast response, ease of access to our scientists.

Difficult projects

We can handle novel or challenging projects other teams may struggle with.

Talk to Our Scientists.

We Have Sequenced 10,000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and developed the first recombinant polyclonal antibody diagnostics.

Unmatched SPR Accuracy and Reproducibility.

Kinetic analysis and validation is performed by our experts, to meet the highest accuracy standards in the industry. We analyze residuals for validation of fit curve accuracy, and conduct advanced biophysical analysis.

Surface Plasmon Resonance on Biacore 1K.

Our latest Surface Plasmon Resonance Biacore instrument is the Biacore 1K, which offers the perfect balance between high-throughput SPR analysis and versatility.

Complex SPR Assays and Difficult Samples.

We work with antibodies and antibody fragments (Fab, scFv), peptide antigens, and large complex protein antigens. With our expertise we can work with novel projects that don’t fit standard workflows.

Expert Analysis and Deeper Insights.

Intense analysis means optimization of binding conditions for every interaction are performed individually for increased accuracy and optimal signal/noise ratio. We provide deeper insights into each interaction with our full regeneration screen using different conditions (acidic, basic, chaotropic and high salt).

On/off rate analysis is presented in an iso-affinity plot to compare the impact of each kinetic parameter on the overall affinity.

Applications of SPR Analysis.

While there are many possible applications of surface plasmon resonance analysis, Rapid Novor is highly focused on antibody research and development. Here are some specialized applications of the technique for the discovery and development stage of antibody R&D:

Antibody Fc Receptor Profiling

These are our specialized kinetic analysis studies for Antibody Fc gamma receptors (FcγRs) and neonatal Fc receptors (FcRn). They provide useful information for prediction of effector functions and serum half-life early in the antibody drug development cycle.

Paratope Mapping

Paratope mapping by mutagenesis is enhanced by measurement with high-throughput SPR. Single amino acid mutations can cause subtle shifts in binding kinetics not detected by simple yes/no binding assays. Examine the binding interface with natural structures in near-biological conditions.

Success Stories.

“The sequences have allowed us to have an “insurance policy” of sorts for critical proteins used in our various products.”

David Ankrapp, Senior Research Scientist, Neogen Corporations

“We’re focused on coupling cytokines with a highly engineered VHVL of an antibody. So by sequencing the antibodies with Rapid Novor we are able to pull out the CDR to graft them into our platform and test in vitro and in vivo.”

Pavel Khrimian – Co-founder and CBO, Deka Biosciences

Getting Started with Surface Plasmon Resonance Services.

Requirements

  • Antibody (100 μg, >90% purity, monoclonal)
  • Antigen (at least 100 μg, >90% purity)
  • Any standard buffer. Inquire about serum analysis.

*Ask us. If your samples don’t meet our requirements, please get in touch. We may be able to work with them.

Deliverables

Full kinetic profile:

  • kon, koff, KD
  • Iso-affinity analysis
  • Optimization and regeneration conditions

High throughput screening:

  • Ranking based on kinetic parameters

Turn-Around Time

Rapid response to calls/emails.

Kinetic Profile – 3 weeks
mAb Screening – 3 weeks
Rush order – 2 weeks

Timing may be sample dependent.

Surface Plasmon Resonance Price.

Custom Kinetic Profile

  • Suitable for detailed analysis of a few interactions
  • Multi-cycle kinetics
  • Optimized conditions per analysis
  • Highly accurate Kon, Koff and KD profile
  • Isoaffinity analysis

Ab-Ag Kinetic Profile

  • Suitable for detailed analysis of a few antibodies
  • Multi-cycle kinetics
  • Optimized conditions per analysis
  • Highly accurate Kon, Koff and KD profile
  • Isoaffinity analysis

Antibody Screening by SPR

  • Suitable for selecting the best antibodies from a larger set
  • Standard conditions
  • Single experiment
  • Binding affinity and off rate screen
  • Lead ranking

Epitope Binning by SPR

  • Suitable for grouping antibodies by the epitope they bind
  • Standard conditions
  • Minimum 3×3 matrix
  • Both orientations tested

*Per sample fees, setup and optimization costs depend on project requirements. Please inquire.

Talk to Our Scientists.

We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics

Talk to our scientists. We have sequenced over 9000+ antibodies and we are eager to help you.

Antibody Sequencing, Discovery and Characterization Services.

Antibody Sequencing & Discovery Services.

REmAb® mAb Sequencing

Monoclonal antibody sequencing from small antibody samples, no need for hybridoma or DNA information. Full sequence in record time.
Explore Antibody Sequencing Services

REpAb® pAb Sequencing

Polyclonal antibody sequencing and antibody discovery service. Sequence antibodies from blood or a polyclonal mixture.
Explore Antibody Discovery Services

RapidSPR™ Analysis

Label-free evaluation of antibody-antigen binding using surface plasmon resonance on Biacore or Nicoya. Can be employed for detailed kinetic profiling, kinetics screening, or epitope binning.

Explore SPR Services

RapidHDX-MS™

Mass spec based epitope mapping. Epitope mapping for identify the binding site of an antibody to its corresponding antigen with the highest confidence and resolution.
Explore HDX-MS Epitope Mapping Service

MATCHmAb™

Antibody verification by rapid peptide mapping. Antibody sequence confirmation and full detailed mapping for development purposes.
Explore Peptide Mapping Service

Antibody Characterization

LC-MS analysis for study of antibody glycosylation, disulfide bonds, post-translational modifications, sequence variants, intact mass and size.
Explore Antibody Characterization Services