Newly published research has identified antibodies capable of preventing malaria, discovered in the blood of a 9-year-old Tanzanian child with previous exposure to the disease. By sequencing antibodies from the child’s blood, researchers identified antibodies that block a critical interaction between the malaria-causing parasite and human blood vessels. Published in PNAS, the study is [...]
To develop robust mAb biologics, it is vital to fully characterize the protein, including its primary sequence, mutations, and important post-translational modifications
To develop robust mAb biologics, it is vital to fully characterize the protein, including its primary sequence, mutations, and important post-translational modifications
We are proud to be part of a newly published peer-reviewed study in Nature Communications. By combining PASEF-enabled mass spectrometry with de novo protein sequencing, the team greatly improved sensitivity and detection in complex microbiomes. The approach introduces the concept of a "druggable metaproteome", offering a new framework to advance microbiome research and develop [...]
Written by: Genya Gorshtein, MSc Published: July 17, 2025 Contents Introduction What is a Linear Epitope? What is a Conformational Epitope? Antigen Design for Antibody Discovery: Linear vs. Conformational Epitope Considerations Protein Interaction Analysis Service with HDX-MS Introduction Antibodies recognize specific regions on antigens [...]
To develop robust mAb biologics, it is vital to fully characterize the protein, including its primary sequence, mutations, and important post-translational modifications
Written by: Genya Gorshtein, MSc Published: June 13, 2025 Contents Introduction What Are Biparatopic Antibodies? How Are Biparatopic Antibodies Engineered? Biparatopic vs. Bispecific Antibodies: What’s the Difference? Engineering Biparatopic Antibodies with Rapid Novor Introduction Biparatopic antibodies are a subclass of bispecific antibodies that bind [...]
Our client is a biotechnology company developing therapeutics that target cancer-specific carbohydrate antigens on the surface of tumour cells.
In this webinar, you will learn: How to efficiently filter out non-specific binders early on to focus only on antibodies with true functional potential. Strategies to isolate highly specific anti-idiotype antibodies that avoid cross-reactivity with natural IgGs. The benefits of combining advanced enrichment techniques with proteomics to discover both blocking (type 1) [...]
Our client is a biotechnology company developing therapeutics that target cancer-specific carbohydrate antigens on the surface of tumour cells.
To develop robust mAb biologics, it is vital to fully characterize the protein, including its primary sequence, mutations, and important post-translational modifications
Written by: Genya Gorshtein, MSc Published: April 9, 2025 Contents Introduction What Is An Anti-Idiotypic Antibody? Applications of Anti-Idiotypic Antibodies in Drug Development Discovery and Generation of Anti-Idiotypic Antibodies Anti-Idiotype Antibody Discovery with Rapid Novor Introduction Anti-idiotypic antibodies are specialized antibodies that bind to [...]
Our client is a biotechnology company developing therapeutics that target cancer-specific carbohydrate antigens on the surface of tumour cells.
Senior Scientist & Manager of HDX-MS Epitope Mapping Services Dr. Dominic Narang is a Senior Scientist & Manager of HDX-MS epitope mapping services at Rapid Novor, bringing over 7 years of experience in structural biology and mass spectrometry. He specializes in providing solutions to biotechnology and pharma companies for their drug discovery and [...]
Written by: Genya Gorshtein, MSc Published: February 10, 2025 Contents Introduction Process of Target Identification and Validation Antibodies as Tools for Target ID and Validation Experiments Custom Antibody Development for Target Validation Custom Antibody Services with Rapid Novor Introduction Successful drug discovery begins with [...]
Our client is a biotechnology company developing therapeutics that target cancer-specific carbohydrate antigens on the surface of tumour cells.
Written by: Genya Gorshtein, MSc Published: December 12, 2024 Contents Introduction B Cells and Their Receptors Antibodies in Serum How Do You Discover Antibodies From Serum? Introduction While B cells play a crucial role in recognizing antigens and initiating immune responses, their differentiation into [...]
Written by: Genya Gorshtein, MSc Published: December 12, 2024 Contents Introduction BCR and Single B Cell Sequencing for Antibody Discovery Antibody Discovery through De Novo Sequencing of Serum Antibodies Proteogenomics: A Complimentary Approach Antibody Discovery With Rapid Novor Introduction B cell sequencing has long [...]
To develop robust mAb biologics, it is vital to fully characterize the protein, including its primary sequence, mutations, and important post-translational modifications
Written by: Genya Gorshtein, MSc Published: October 16, 2024 Contents Introduction Epitope Mapping Methods Addressing the Limitations of HDX-MS for Epitope Mapping HDX-MS as a Stand Alone Method for Epitope Mapping Epitope Mapping with HDX-MS at Rapid Novor Introduction Understanding antibody-antigen interactions is critical [...]
To develop robust mAb biologics, it is vital to fully characterize the protein, including its primary sequence, mutations, and important post-translational modifications
Rapid Novor Inc., the world's leader in mass spectrometry (MS)-based antibody sequencing, published a peer-reviewed research article where REpAb® antibody discovery platform was used to sequence a complex mixture of functional antibodies directly from the serum of a human patient after receiving a COVID-19 vaccine. Titled “De Novo Protein Sequencing of Antibodies for Identification [...]
In this webinar, you will learn: How mAb irreproducibility could unknowingly impact your research outcomes Proven strategies to mitigate reproducibility risks when using research reagents The role of antibody sequencing in ensuring reproducibility with mAbs Rapid, cost-effective sequence confirmation for routine applications The next frontier: polyclonal antibody sequencing and recombinant pAbs [...]
Written by: Genya Gorshtein, MSc Published: August 26, 2024 Contents Introduction Advantages of Antibody Discovery In Diverse Species Unique Features of Antibodies from Diverse Hosts Antibody Discovery Platforms for Diverse Hosts Introduction The historical use of mice for monoclonal antibody generation has been driven [...]
Written by: Genya Gorshtein, MSc Published: August 8, 2024 Contents Introduction What are Protein Sequence Variants Impact of Sequence Variants on Monoclonal Antibodies Challenges in Sequence Variant Analysis and Detection Sequence Variant Analysis Service Introduction Sequence variants are unwanted impurities in antibody and biotherapeutic [...]
Principal Scientist in Polyclonal Antibody Sequencing Dr. Thierry Le Bihan joined Rapid Novor as a Principal Scientist in 2018, tasked with leading a multidisciplinary team to develop a polyclonal antibody sequencing platform. Thierry brought extensive proteomics and mass spectrometry expertise from his previous roles as Director of Proteomics at The Campbell Family Institute [...]
This paper is now published in Nature Communications! Le Bihan, T., Nunez de Villavicencio Diaz, T., Reitzel, C. et al. De novo protein sequencing of antibodies for identification of neutralizing antibodies in human plasma post SARS-CoV-2 vaccination. Nat Commun 15, 8790 (2024). https://doi.org/10.1038/s41467-024-53105-8 In this webinar, you will: Explore how using a [...]
To develop robust mAb biologics, it is vital to fully characterize the protein, including its primary sequence, mutations, and important post-translational modifications
Written by: Genya Gorshtein, MSc Published: June 10, 2024 Contents Introduction Alanine Scanning Mutagenesis Epitope Mapping HDX-MS Epitope Mapping Antibody Epitope Mapping Services Introduction Epitope mapping elucidates the antibody function by identifying specific binding sites on the antigen that interact with the antibody. Alanine [...]
In this webinar, you will learn: How AI and HDX-MS are applied in screening, epitope mapping, and selecting neutralizing antibodies against SARS-Cov-2. The predictive capabilities of AI computational modeling and its validation and refinement by high-resolution HDX-MS. How the integration of AI with HDX-MS informs antibody characterization for the accelerated development of therapeutic [...]
Written by: Genya Gorshtein, MSc Published: May 29, 2024 Contents Introduction What is Epitope Binning Value of Epitope Binning in Early Discovery Stages Epitope Binning with SPR on Biacore 1K Introduction An epitope is an inherent property of an antibody that cannot be altered [...]
To develop robust mAb biologics, it is vital to fully characterize the protein, including its primary sequence, mutations, and important post-translational modifications
A Canadian Multiple Myeloma (MM) clinical trial sponsored by Rapid Novor, has enrolled its first patient. This is the first Canadian prospective clinical study aiming to evaluate the EasyM™ assay (mass spectrometry measurement of a myeloma biomarker) for disease response in newly diagnosed MM patients (NDMM) receiving standard of care treatment. A clinical [...]
In this webinar, you will… Learn the details of LC-MS based antibody characterization in early discovery and production pipelines Explore in-depth the analytical methodologies and strategies essential for comprehensive antibody characterization, covering post-translational modifications, glycan profiles, peptide mapping, and disulfide linkages Ask your burning questions and get expert guidance on navigating antibody [...]
Written by: Genya Gorshtein, MSc Published: April 19, 2024 Contents Introduction Bispecifics and Multispecific Antibody Formats Mechanism of Action of Bispecific Antibodies Bispecific and Multispecific Development with Rapid Novor Introduction The complex nature of diseases often limits the therapeutic efficacy of monovalent antibodies. To [...]
To develop robust mAb biologics, it is vital to fully characterize the protein, including its primary sequence, mutations, and important post-translational modifications
Written by: Genya Gorshtein, MSc Published: April 3, 2024 Contents Introduction Membrane Proteins are Difficult Antigens Strategies for Utilizing Membrane Proteins as Antigens in Antibody Discovery In Vivo Antibody Discovery Using Mass Spectrometry Introduction Membrane proteins orchestrate a wide range of cellular functions, such [...]
To develop robust mAb biologics, it is vital to fully characterize the protein, including its primary sequence, mutations, and important post-translational modifications
Rapid Novor Inc and MAbSilico announced that they have partnered to provide the world’s first AI-driven HDX-MS epitope mapping service for antibody development. By seamlessly integrating experimental data from HDX-MS with predictive analytics derived from AI-driven computational modeling, researchers can gain a comprehensive understanding of antibody structure, dynamics, and interactions with unparalleled precision and [...]
To develop robust mAb biologics, it is vital to fully characterize the protein, including its primary sequence, mutations, and important post-translational modifications
Written by: Genya Gorshtein, MSc Published: January 8, 2024 Contents Introduction Glycoengineering of Therapeutic mAbs Glycoengineering Methods Informed Glycan Engineering through LC-MS Glycan Analysis Introduction Glycosylation plays a crucial role in antibody functions, influencing protein folding, trafficking, stability, and half-life. While the glycosylated residue [...]
Written by: Jenna Kerry, MSc Published: October 25, 2023 Contents Introduction What is Affinity Maturation? How Does Somatic Hypermutation Work? Applications of Affinity Maturation De Novo Proteomic Sequencing of Antibodies Introduction The immune system is a complex system responsible for protecting organisms from various [...]
Written by: Genya Gorshtein, MSc Published: October 18, 2023 Contents Introduction Types of Glycans How are Proteins Glycosylated? Why are Proteins Glycosylated? Protein Glycosylation in the Immune System Importance of Understanding Glycan Profiles of Proteins Discover Glycan Sites and Profiles with Rapid Novor Introduction [...]
In this webinar, you will learn: Workflows for antibody characterization that extend beyond the primary amino acid sequence How to leverage HDX-MS for epitope mapping and SPR for binding kinetics to understand the mechanism of action and maximize the effectiveness of your characterization efforts How to ensure high-quality antibody production with [...]
Written by: Genya Gorshtein, MSc Published: September 12, 2023 Contents Introduction Mechanisms That Influence Protein Aggregation and Stability Sequence and Structural Characteristics That Impact Antibody Aggregation and Stability Engineered Antibody Formats Influence Protein Aggregation Strategies to Reduce Antibody Aggregation Propensity Improving Antibody Developability with Next Generation Protein Sequencing and Proteomics [...]
Our client is a biotechnology company developing therapeutics that target cancer-specific carbohydrate antigens on the surface of tumour cells.
Our client is a biotechnology company developing therapeutics that target cancer-specific carbohydrate antigens on the surface of tumour cells.
Written by: Genya Gorshtein, MSc Published: June 13, 2023 Contents Introduction How do Primary and Secondary Antibodies Work? How are Primary and Secondary Antibodies Generated? Selecting Primary and Secondary Antibodies for Immunoassays Developing Reliable Reagents for Immunoassays with Rapid Novor Introduction Immunoassays have a [...]
Written by: Genya Gorshtein, MSc Published: May 9, 2023 Contents Introduction Hybridoma Instability Leads to mAb Irreproducibility Batch-to-Batch Variation in Polyclonal Antibodies Antibody Sequencing and Recombinant Expression Ensures Reproducible Antibody Reagents Generating Reproducible Reagents with Rapid Novor Introduction Antibodies (Abs) are indispensable tools in [...]
Written by: Genya Gorshtein, MSc Published: April 21, 2023 Contents Introduction Target Specification for First Generation Antibodies Patent Strategies for Second-Generation Antibodies Next Generation Protein Sequencing and Proteomics Strengthens IP Protections Introduction Intellectual property (IP) protection is a critical step during the commercialization of antibodies [...]
Written by: Genya Gorshtein, MSc Published: February 17, 2023 Contents The Drug Development Pathway Target Discovery and Validation Antibody Discovery Characterization and Lead Selection Engineering and Optimization Candidate Selection Therapeutic Antibody Discovery with Rapid Novor The Drug Development Pathway The four phases in the drug [...]
Written by: Genya Gorshtein, MSc Published: January 6, 2023 Contents Introduction What is Affinity? What is Avidity? Measuring Affinity and Avidity: ELISA or SPR? Measuring Affinity And Avidity For Biological Applications Affinity and Avidity Analysis via SPR with Rapid Novor Introduction Kinetics of antibody-antigen interactions [...]
Written by: Genya Gorshtein, MSc Published: November 1, 2022 Contents Introduction Approaches for Engineering Antibody Therapeutics Driving Antibody Engineering with Next Generation Protein Sequencing and Proteomics Introduction Antibody engineering encompasses various development, production strategies, and modification techniques to improve the biological properties of monoclonal antibodies (mAbs) [...]
Written by: Genya Gorshtein, MSc Published: November 25, 2022 Contents Introduction General PROTAC Structure and Function Antibody-Conjugated PROTACs Developing AbPROTACs with De Novo Antibody Sequencing and Proteomics Introduction Small-molecule drug development is aimed at inhibiting disease-promoting protein function through occupancy-driven protein inhibition. A major caveat of [...]
Written by: Genya Gorshtein, MSc Published: September 14, 2022 Contents Introduction ADCs as Novel Anti-Cancer Chemotherapeutics Key Components of ADCs Future Generation of ADCs De Novo Protein Sequencing Applications in ADC Development Introduction An antibody-drug conjugate (ADC) is a monoclonal antibody (mAb) with a covalently attached [...]
Written by Genya Gorshtein, MSc Updated: January 27, 2023 (Published: August 31, 2022) Contents How is Antibody Diversity Generated? Which Steps Contribute to the Generation of Antibody Diversity? Antibody Loci and V(D)J Recombination Somatic Hypermutation Class Switch Recombination De Novo Proteomic Sequencing of Antibodies How is Antibody [...]
Written by: Genya Gorshtein, MSc Published: August 18, 2022 Contents Introduction How are Polyclonal Antibodies Produced? Applications of Polyclonal Antibodies Challenges of Polyclonal Antibodies De Novo Polyclonal Antibody Sequencing Introduction Polyclonal antibodies (pAbs) are a heterogeneous mix of antibodies derived from B cells in the [...]
Written by Genya Gorshtein, MSc August 10, 2022 Contents General Structure of Antibodies Fab and Fc Fragments Hinge Region CDR and FR Regions Antibody Structure Analysis Services General Structure of Antibodies Antibodies or immunoglobulins (Ig) maintain a common quaternary structure consisting of two identical heavy chains (HCs) and [...]