Antibody Fc Receptor Profiling.

Predict Effector Function and Pharmacokinetics with High-Precision Biacore SPR Analysis.

What is Fc Receptor Profiling?

Fc receptor profiling involves the quantitative measurement of the interaction between the Fc (fragment crystallizable) region of an antibody and various cell surface receptors. These interactions are the primary drivers of an antibody’s biological activity beyond antigen binding. By characterizing the binding kinetics (kon, koff) and affinity (KD), researchers can determine how effectively an antibody will trigger immune responses or how long it will remain circulating in the body.

Why is Fc Receptor Profiling Essential for Antibody Development?

Characterizing the Fc region is a regulatory and functional requirement for biotherapeutic development. It allows for the fine-tuning of an antibody’s profile to match its intended therapeutic use:

  • Effector function prediction
  • Pharmacokinetics and Half-Life
  • Fc Silencing and Effector Function Knockout
  • Safety and Efficacy

How Fc Receptor Profiling works.

Rapid Novor provides comprehensive Fc receptor (FcR) profiling through our RapidSPR™ platform, utilizing the industry-leading Biacore 1K system and our comprehensive Fc receptor panels.

Our specialized binding assays for Fc gamma receptors (FcγRs) and neonatal Fc receptor (FcRn) offer critical insights into the therapeutic potential of your antibody candidates, enabling the prediction of effector functions and serum half-life early in the development cycle.

We utilize the Biacore 1K SPR system to perform high-sensitivity, label-free analysis of these interactions, ensuring that even low-affinity binding events are characterized with precision.

RapidSPRTM Comprehensive Receptor Panels.

Our RapidSPR™ service covers the full spectrum of relevant Fc receptors. We offer broad panel screens, or more focused cohorts if you just have a couple of candidates. We can provide panels, or adapt to your custom needs.

Below are typical targets featured within our panel offerings. While these represent common setups, our platform accommodates any custom target sequence required for your project. We can work with our trusted vendors on your behalf to order fresh panels at the start of each project.

 

Receptor Category Specific Receptors Predicted Biological Function
High-Affinity FcγR CD64 (FcγRI) Potent trigger of ADCP and pro-inflammatory cytokine release.
Low-Affinity FcγRs CD16 (FcγRIIIa/b), CD32 (FcγRIIa/b/c) Drivers of ADCC (CD16) and inhibitory/activatory immune signaling.
Salvage Receptor FcRn Regulation of IgG and albumin homeostasis; determines serum half-life.

RapidSPRTM Biacore Platform.

Surface Plasmon Resonance (SPR) is the “gold standard” for biomolecular characterization. While other methods like ELISA or Bio-Layer Interferometry (BLI) provide endpoint data, Biacore SPR offers clear advantages:

Real-Time Kinetics

We provide full kinetic profiles including association (kon, ka) and dissociation (koff, kd) rates, as well as affinity constants (KD).

High Sensitivity

The Biacore 1K platform offers unmatched sensitivity with extremely low baseline noise, making it ideal for detecting weak interactions such as those involving FcRn or low-affinity FcγRs.

Label-Free Analysis

Interactions are measured in their native state without the need for fluorescent or radioactive tags that could interfere with binding.

Scientific Rigor

Our SPR experts employ robust kinetic modeling personally tailored to your specific interaction, with detailed documentation of our curve-fitting parameters, residuals, and quality checks to ensure data transparency and reliability.

Platform superiority

Unlike BLI, which risks dipping below detection limits and losing weak or fast-dissociating binders (such as physiological FcRn at neutral pH or low-affinity FcγRII/III variants), our high-sensitivity SPR configuration reliably captures and quantifies transient, fast-kinetic interactions without risk of false negatives.

The Rapid Novor Difference:

Complex Half-Life Analysis.

All FcRn projects come with custom half-life analysis.

  • Our custom analysis measures the exact time required for half the antibody-FcRn complex to dissociate (t1/2), giving a clear readout of residence time at pH 6.0 versus at pH 7.4.
  • Complex half-life is a better predictor of antibody clearance in vivo than a standard affinity ranking, allowing you to confidently identify and advance top candidates.

Getting Started.

Requirements

Talk to us for exact requirements, which can change depending on your project. Below are some general guidelines.

Antibodies:

  • Minimum 500 µg mAb
  • 90% purity (determined by SDS-PAGE or SEC)

[For optional Fc receptor(s), in place of or in addition to our panels:]

  • Minimum 100 µg per mAb
  • 90% purity

Deliverables

  • Full kinetic analysis
  • Fit curves
  • Residuals
  • Iso-affinity plots for comparative ranking
  • Complex half-life analysis (residence time) to determine precise clearance rates for your antibody candidates

Turn-around time

4 to 5 weeks from sample QC approval to final report delivery.

Talk to Our Scientists.

We Have Sequenced 10,000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and developed the first recombinant polyclonal antibody diagnostics.

Talk to Our Scientists.

We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics

Talk to our scientists. We have sequenced over 9000+ antibodies and we are eager to help you.

Antibody Sequencing, Discovery and Characterization Services.

Antibody Sequencing & Discovery Services.

REmAb® mAb Sequencing

Monoclonal antibody sequencing from small antibody samples, no need for hybridoma or DNA information. Full sequence in record time.
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REpAb® pAb Sequencing

Polyclonal antibody sequencing and antibody discovery service. Sequence antibodies from blood or a polyclonal mixture.
Explore Antibody Discovery Services

RapidSPR™ Analysis

Label-free evaluation of antibody-antigen binding using surface plasmon resonance on Biacore or Nicoya. Can be employed for detailed kinetic profiling, kinetics screening, or epitope binning.

Explore SPR Services

RapidHDX-MS™

Mass spec based epitope mapping. Epitope mapping for identify the binding site of an antibody to its corresponding antigen with the highest confidence and resolution.
Explore HDX-MS Epitope Mapping Service

MATCHmAb™

Antibody verification by rapid peptide mapping. Antibody sequence confirmation and full detailed mapping for development purposes.
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Antibody Characterization

LC-MS analysis for study of antibody glycosylation, disulfide bonds, post-translational modifications, sequence variants, intact mass and size.
Explore Antibody Characterization Services