Research Challenges in Veterinary Medicine
Since 2006, the One Health Initiative (OHI)’s goal has been to demonstrate the inextricable link between humans, animals, and the environment. Certainly, the current global pandemic is a great testament to the ties between climate change, humans, and animals that OHI has been working to highlight. The rise of other zoonotic diseases (e.g., Hendra, and Nipah viruses) not only directly affect humans by transmitting disease but may also result in deep impacts on the food supply. Likewise, the rise of antibiotic resistance is a great threat affecting both human and animal lives. Policies have been put in place since the 2000s by the FDA and Health Canada to curve antibiotic overuse, especially in farming practices. Moreover, as the world’s human and animal populations grow, the demand for products rises, and with it the need for waste management and toxicology surveillance. The latter becomes ever-pressing in animal agriculture as production animals are often the first to show symptoms from toxin exposure. Finally, developed countries have seen the lifespan of companion animals significantly increase in the last decade alone, and the trend is expected to continue. With a lengthier lifespan, the propensity for neoplastic diseases and comorbidities also rises. Because the quality of owner care has drastically improved, there is a demand for better therapeutics and diagnostics, particularly those of non-invasive nature.
The Root Cause of These Challenges
However, the lack of commercially available and validated antibody reagents across many species continues to undermine progress in veterinary medicine and research. With a limited understanding of other species’ immune systems, and few species having their whole genomes sequenced, it’s difficult to identify disease targets for biomarkers, let alone produce the antibody reagents necessary for the study of species. As such, model organisms are typically relied on, specifically mice, without little overlap or relevance to the target species. Current immunological reagents are discovered via methods reliant on nucleotide sequencing; however, the latter lack depth of knowledge of the circulating antibody repertoire due to missing relevant information on the primary amino acid structure that includes post-translation modifications data.
Next Generation Protein Sequencing Solution
Recombinant monoclonal antibodies (rAbs) derived from the target animal’s circulating antibodies through polyclonal antibody protein sequencing (REpAb®) offer a solution to the aforementioned problems. Using Rapid Novor’s REpAb® Antibody Discovery Platform, pAb sequences can be mined from the naturally occurring humoral response. Such rAbs would be strictly defined and bear high fidelity. REpAb® could also be used to generate more precise animal-derived pAb positive controls for immunoassays such as anti-drug antibody assays needed to evaluate therapeutics for companion animals. With REpAb®’s technology, scientists can confidently rely on new and improved biomarkers, therapeutics, and diagnostics to ease the characterization of the adaptive immune response in all animals.
What You Will Learn
- How Next Generation Protein Sequencing (NGPS) can be used in zoonotic infection control
- How clients have used our services in the past to curb antimicrobial resistance
- How a similar approach can be used for toxin surveillance
- What a better anti-drug antibody solution would look like using REpAb®
- How protein sequencing-based liquid biopsy screens can sensitively and specifically monitor neoplastic diseases like multiple myeloma in animals
- More about our NGPS workflow, and how it can complement existing pipelines in veterinary research
Director of International Business Development at Rapid Novor
Anthony works with leading researchers in antibody discovery and diagnostic development to understand their needs and ensure that REpAb® polyclonal antibody sequencing technology complements existing approaches and/or provides a complete end-to-end solution. Anthony is dedicated to solving challenging technical problems in the biomedical field while enriching the lives of humans around the world.