What is igBLAST?
IgBLAST is a sequence analysis tool for antibody variable domain sequences. It matches matches to the germline V, D and J genes, details at rearrangement junctions, the delineation of IG V domain framework regions and complementarity determining regions (CDR). Understanding the sequence of your antibody is an important, and often critical step in antibody research. igBLAST provides a useful way to compare your antibody sequence to reference sequences.
Options for Antibody Sequencing and igBLAST
To determine the antibody’s sequence, you have two options:
- DNA sequencing techniques can be used, if you have access to the cell-line/hybridoma, to determine the antibody sequence
- You can use antibody sequencing (a.k.a. Antibody Protein Sequencing) techniques to sequence your antibody if you just have an antibody sample or you wish to understand post-translational modifications, phosphorylation, glycosylation, etc.
In both cases, igBLAST can be used in post-analysis.