Antibody Protein Sequencing Service
Antibody protein de novo sequencing technology can determine the amino acid sequence of an unknown antibody with mass spectrometry. Antibody protein sequencing does not require access to the cell line or prior knowledge of the DNA sequence. The technology is based on mass spectrometry measurements of the protein molecules. With merely 0.1mg of the antibody protein, within weeks you can receive a detailed report with the accurate heavy and light chain antibody sequences.
Key Benefits – Antibody Sequencing Service
Unparalleled Antibody Sequencing Accuracy
With our Antibody Sequencing Service, the identity of each amino acid in the antibody sequence is supported by strong signal peaks in multiple MS/MS spectra of distinct peptides so the complete amino acid sequence can be determined with confidence. Additionally, post-translational modifications can be characterized and understood – leading to an understanding of the antibody which would not be possible if only the DNA sequence is known.
Accurate Ile/Leu determination
Unlike other Antibody Sequencing Services, our REmAb® + WILD® method can distinguish isomeric residues such as Isoleucine and Leucine. This removes the guess work in the Ile/Leu determination by the traditional enzyme specificity method. This greatly reduces the alternative forms one has to try during expression, and thus saves time and money.
Our Antibody Sequencing Service works for all antibody types (IgG, IgA, IgM, IgY, Fab, ScFv, etc.) and species (human, mouse, rat, rabbit, hamster, sheep, goat, etc). We have also frequently sequenced antibodies with multiple different light chains as well as conjugated and contaminated proteins. Our antibody sequencing service will work for your antibody.
|Choose Your Package|
- Requires only very small sample
- Full sequence from n-term to c-term
- Works on all antibody types and species
- Report on heavy and light chain full sequences
- Raw MS/MS data provided on request
- Ile/Leu inferred by Enzyme specificity only
- Ile/Leu determined by WILD™ technology
- Recombinant expression of 3 mg antibody proteins in IgG format
- Money back guarantee (see details below)
|REmAb™ with WILD™ and Expression|
- 9 weeks
|REmAb™ with WILD™|
- 2 weeks
- 3 weeks