November 18, 2025 | 10:00AM EST
In this webinar, you will learn:
- Explore how REpAb can sequence antibodies from the plasma of a naturally exposed human, identifying functional antibodies in circulation.
- Review study findings showing antibodies with broad inhibitory activity against diverse malaria variants, validated through structural and functional analysis.
- Learn how REpAb can be applied to study and identify protective antibodies against infectious diseases.
Malaria Antibody Discovery
Not all binders are created equal, and sequencing them all is inefficient. A test-then-express approach eliminates non-specific binders early in the workflow, saving time and increasing the likelihood of identifying true functional hits.
In this webinar, we’ll explore how functional pre-selection strategies streamline anti-idiotype antibody discovery by enriching only the binders that matter.
The main challenge in anti-idiotypic antibody discovery is finding antibodies that are highly specific to the therapeutic antibody, without cross-reacting with naturally circulating IgGs. Our workflow leverages advanced protein enrichment and depletion strategies to isolate functionally relevant anti-idiotype subtypes before sequencing, significantly boosting discovery efficiency.
Using this targeted enrichment approach, we produced polyclonal antibodies with lower off-target binding to human IgG than commercially available anti-idiotypes. Recombinantly expressed antibodies derived from these pools showed high affinity for the adalimumab target, and no/low affinity for human IgG in general. The method also supports discovery of both blocking and non-blocking anti-idiotypes through integrated proteomics and functional selection approaches.
Speaker Bios
Thomas Lavstsen, PhD
Principal Investigator at the Centre for Translational Medicine and Parasitology; University of Copenhagen.
Teresa Nunez de Villavicencio Diaz, PhD
Senior Scientist, Polyclonal Antibody Sequencing; Rapid Novor