Webinar Highlights

You will learn:

  • Introduction to the structure and function of the tuberous sclerosis protein complex (pTSC)
  • A novel strategy for isolating and purifying protein complexes from native sources using recombinantly produced Fabs
  • How Next Generation Protein Sequencing (NGPS) works
  • Applications of NGPS to aid design and engineering of recombinant antibody fragments

Abstract

The tuberous sclerosis protein complex (pTSC) is an intracellular stress-sensor that nucleates a proteinaceous signaling hub to integrate regulatory information about the growth and energy consumption of the cell. The structure and stoichiometry of pTSC have been revealed through previous biochemical and cryo-electron microscopy (cryo-EM) studies, hinting at the possibility of pTSC forming large higher-order oligomers. However, these studies required the stabilization of the protein with chemical cross-linking or graphene oxide-coated grids during sample preparation, making it difficult to obtain a complete understanding of its architecture.

Though there has been immense progress in filling information gaps, there is still much to be learned with regards to understudied and/or unstable proteins and protein complexes, like pTSC. One of the major challenges that researchers often face is the effort to express, purify, and isolate proteins for downstream structural characterization. Purification and expression of recombinant proteins remain the preferred method for obtaining protein samples; however, this can be a demanding task for certain proteins.

Protein complexes may form higher-order structures only when they are expressed under native conditions within the host organism. Therefore, novel techniques for isolating proteins from their native sources allow for progress in characterizing those unstable, ‘tricky’ proteins. Next generation protein sequencing (NGPS) is one approach that is being leveraged in protein purification strategies. With NGPS, the primary amino acid sequences of commercially available mAbs are now being derived and utilized to engineer recombinant antibodies and antibody fragments that show improved target specificity for capturing proteins and protein complexes from their native sources. Strategies like these can help provide the structural and functional information towards our understanding of diverse proteins and their intricacies.

Speaker Bios

Webinar

Kinetic Characteristization of Alpaca Monoclonal Antibodies

Nov 30th, 11 am EST

All Things Proteomics Newsletter.

Talk to Our Scientists.

We Have Sequenced 7000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and developed the first recombinant polyclonal antibody diagnostics.

Talk to Our Scientists.

We Have Sequenced 6000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics

Talk to our scientists. We have sequenced over 6000 antibodies and we are eager to help you.