Study: Increased De Novo Protein Sequencing Coverage with Optimal Protease Cocktail

Overview Introduction Sample preparation for complete LC-MS/MS sequencing of a pure protein sample differs substantially from the preparation used for protein identification in complex samples. To sequence a protein de novo, the experimental design should aim to identify each amino acid (a.a.) multiple times within different peptides, instead of relying on only a few peptides [...]

2019-08-12T15:02:52-04:00August 8th, 2019|Research|0 Comments

Study: De Novo Protein Sequencing Vs. DNA Sequencing

A Large-Scale Comparison of MS-based Antibody De Novo Protein Sequencing and Targeted DNA Sequencing Introduction The DNA sequences of antibodies are highly diverse due to the V-(D)-J recombination and hypersomatic mutations. As such, a new antibody of interest is unlikely to appear in any existing sequence database. Consequently, the database search approach commonly used in [...]

2020-01-06T15:33:16-05:00June 7th, 2019|Research|5 Comments

Study: Prevalence of Secondary Light Chains

Introduction Monoclonal antibodies (mAbs) are widely used in research, diagnosis, and pharmaceutical purposes. Lately, the relatively lower quality of the research-purpose mAbs is a point of concern within the research community¹,². The problems include the lack of validation and low reproducibility. As most of the validation is done by immunoassay, the extent of protein contamination, [...]

2019-12-04T12:01:20-05:00February 5th, 2019|Research|0 Comments

Novor Paper Published @ JASMS

The paper, Novor: Real-Time Peptide de Novo Sequencing Software, is published. It is open access and can be downloaded here. At Rapid Novor, we believe technologies only get better when more people can access them. Thus, we provide Novor software Free of charge for academic research. Compared with the state-of-the-art, Novor significantly improved the de [...]

2016-10-17T14:21:56-04:00July 6th, 2015|Research|0 Comments