August 2019

Protein Sequencing & Antibody Species

2021-11-18T11:07:25-05:00August 26th, 2019|Articles|

We are able to sequence any species because we use de novo protein sequencing by tandem mass spectrometry (MS/MS). This type of protein sequencing by mass spectrometry does not rely on existing databases that might introduce bias or wrong amino acid calls.

Protein Sequencing and Protein Purity

2021-11-18T11:06:16-05:00August 12th, 2019|Articles|

How much protein and what purity is needed for protein sequencing? A: We typically accept samples at 100 µg with at least 80% purity, rare in the field. To measure the amount of protein, you can use a microvolume spectrophotometer such as the Nanodrop or a traditional Bradford assay.

Protein Sequencing and Contaminants

2021-11-18T11:05:47-05:00August 5th, 2019|Articles|

The most common protein contaminants include keratin and serum albumin. Other Mass Spectrometry (MS) protein contaminants identified in research labs worldwide by a recent Human Proteome Organization study are casein and E. coli proteins.