Antibody Sequencing Services2023-06-07T10:37:31-04:00

REmAb® de novo Antibody Sequencing by Mass Spectrometry.

Our antibody sequencing service determines the amino acid sequence of monoclonal antibodies with our proprietary high-throughput, full coverage de novo antibody sequencing technology.

How REmAb® de novo Antibody Sequencing Works.

How It Works.

The REmAb® de novo antibody sequencing workflow delivers the complete antibody sequence, including CDRs, with unparalleled accuracy and reliability. Starting with just 100μg of purified antibody, the sample undergoes multi-enzyme digestions and the fragments are measured with LC-MS/MS to derive the de novo peptide sequences. These fragments are then analyzed by Rapid Novor’s suite of bioinformatics algorithms to assemble the full-length antibody sequences of any isotype.

Our unique de novo workflow allows us to determine the full antibody sequence with hundreds of overlapped peptides without requiring a database or sequence library for reference or homology.

monoclonal antibody sequencing workflow through de novo protein sequencing

The REmAb® Advantage.

REmAb
Highest throughput: 50 to 100 mAbs per week
Highest accuracy: 100%
Lowest sample requirement: 50 μg
Most enzymes: 5-10 digests
Proprietary, next generation algorithms
Ile/Leu calls with WILD and WILDtag technology
Ability to handle the unexpected
Highest coverage: 100X
One sequence that works, every time
REmAb® Others
Throughput 50 to 100 mAbs per week Single runs
Accuracy 100% Often low / ambiguous
Sample volume requirement 50 μg (as low as 7μg) >150 μg
Multi-enzyme digest At least 5 – 10 enzymes 1 or 2
Bioinformatics software Proprietary, next generation algorithms Previous generation commercial software
Accurate Ile/Leu Determination Yes, WILD® and WILDtag technology Rarely
Ability to handle the unexpected Yes: additional chains, impurities, oligos, etc. Rarely
Depth of coverage 100X Low or patchy
Downstream impact One sequence that works, every time Costly ambiguities to express and test

WILD® Isoleucine-Leucine Determination.

Isobaric combinations with confidence: our patented WILD® method was the first commercial service to use additional peptide fragmentation via electron transfer high collision energy dissociation (EThcD) to create and analyze w-ions. This enables true Isoleucine-Leucine determination based on experimental evidence alone. Our unique and patented WILDtag chemistry increases coverage and confidence in isobaric assignments.

WILD Isoluecine-Leucine determination allows us to obtain 100% accurate protein sequence with highest industry coverage

De novo Sequencing of Any Isotype from any Species.

Our unique de novo workflow allows us to determine the full antibody sequence without requiring a database or sequence library for reference or homology. We’ve worked with all isotypes and popular constructs, including bispecific, chimeric antibodies, nanobodies and fAbs. Secure 100% coverage of variable domains and obtain antibody sequences that recognize the right antigen with de novo sequencing service.

We Are the World Leading Experts in de novo Antibody Sequencing.

Our team comprises of experienced leaders in mass spectrometry proteomics, protein chemistry and bioinformatics. Even if your sample doesn’t meet our minimum requirements, come talk to us. REmAb® de novo antibody sequencing is an important tool for scientists in sequence analysis, particularly for the identification and characterization of novel antibodies.

Success Stories.

0
sequencing projects completed since 2015. Here’s what our customers said 2-3 months after the project:
Happy

100% of respondents were happy with the experience

Refer

Respondents were 9.7/10 likely to refer their peers.

Early wins

85% were already successful downstream. Remainder were in progress. No reported failure.

“You are certainly the best CRO we’ve ever worked with!❤️”

Roya Servattalab, Scientist II, Marengo Therapeutics

“We’ve focused on coupling cytokines with highly engineered VHVL of an antibody. So by sequencing the antibodies with Rapid Novor we are able to pull out the CDR to grant them into our platform and test in vitro and in vivo”

Pavel Khrimian, Co-Founder & CBO, Deka BioSciences

“Rapid Novor was tasked with finding highly specific, high affinity antibodies against an important target – and they delivered powerful antibodies fit-for-purpose. Such antibodies are not easily obtained yet Rapid Novor approach proved immediately successful, despite our time-constrained and stringent demands”

Matt Pope, Principal Scientist, SISCAPA

Getting Started.

Requirements.

  • >50 μg of monoclonal antibody
  • >80% purity
  • Any isotype from any species

Is your antibody sample smaller or less pure?Ask us. We’ve sequenced many mAbs when the sample amount was much less than our requirements.

Deliverables.

  • Complete antibody sequence in FASTA
  • Interactive peptide coverage viewer and report
  • Notable observations

Turn-Around Time.

Rush, 1 week
1 sample, 2 wks
2-10 samples, 3 weeks
11+ samples, 4 weeks

Antibody Sequencing Pricing.

First Time Client

$4,999Over 60% OFF
  • Promo for IGg only

Regular Price

$13,999
  • Bulk Order Discounts Available

Optional Add Ons.

Talk to Our Scientists.

We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and developed the first recombinant polyclonal antibody diagnostics.

Talk to Our Scientists.

We Have Sequenced 9000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics

Talk to our scientists. We have sequenced over 9000+ antibodies and we are eager to help you.

REmAb® de novo Antibody Sequencing Applications.

Other Protein Characterization Services.

Antibody Sequencing & Discovery Services.

REpAb®

Polyclonal protein sequencing and antibody discovery service. Sequence antibodies from blood or a polyclonal mixture.
Explore REpAb®

SPR Analysis

Label-free evaluation of protein-antigen binding relies on a Surface Plasmon Resonance technology. Combined with our expertise in protein interactions, this results in high sensitivity and the lowest noise even in complex analytes.
Explore SPR Analysis

HDX-MS

Mass spectrometry-based epitope mapping identifies the binding site of a protein or compound to its corresponding antigen. Our state-of-the-art instrumentation enables this analysis with the highest confidence and resolution.

Explore HDX-MS