Antibody Protein Expression
Antibody Protein Expression is a special case of protein expression that produces Antibody proteins. The antibodies resulting from Antibody Expression are often called monoclonal antibodies (mAb), since they are all cloned and should all be identical in sequence. Antibodies can of course be produced from Hybridoma, but mAb have advantages.
Protein Sequencing followed by Expression
In some research or pharmaceutical applications, it is necessary to express or produce more of a protein or antibody sample, but the DNA sequence of the protein/antibody is not yet known. In other cases, the sequence of the protein or antibody is only partially known with confidence. In these cases, Protein de novo Sequencing can be used to determine the sequence of the protein or antibody directly from the sample without reference to a DNA sequence, thus enabling expression.
Sequencing to Reducing the Cost and Complexity of Protein Expression
If the sequence of a desired protein or antibody is not fully known prior to expression, this can result in prohibitive time and materials costs to express, purify and concentrate the right protein for downstream use.
For instance, if one amino acid could be swapped for another, researchers would have to express two variants of the protein or antibody. Downstream testing would confirm which sequence produced the protein or antibody with the desired characteristics. If two amino acids could be swapped, this would result in 4 combinations to be expressed and tested. Three amino acids swapped could mean 15 sequences to express and test.