About Maria Gerpe

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So far Maria Gerpe has created 22 blog entries.

October 2021

Ushering the New Era in Anti-Drug Antibody Assays with Next Generation Protein Sequencing

2021-10-15T13:30:54-04:00October 15th, 2021|Whitepapers|

Anti-drug antibody (ADA) assays are critical to assess the clinical efficacy and safety of a biological drug and rely on control reagents that mimic the ADA response to the biological drug being tested. These positive controls typically consist of animal-derived pooled polyclonal antibodies or human monoclonal antibody reference panels against the target protein drug.

September 2021

De Novo Protein Sequencing

2021-10-05T08:01:38-04:00September 28th, 2021|Webinars|

Abstract In this on-demand webinar, we briefly cover the fundamentals of protein sequencing, how researchers have benefited from implementing protein sequencing into their pipelines, and discuss how Rapid Novor is able to routinely and robustly achieve 100% accuracy and 100% coverage for both monoclonal and oligoclonal antibodies. Webinar details [...]

Antibody Characterization by Mass Spectrometry

2021-10-05T08:03:18-04:00September 28th, 2021|Webinars|

Abstract In this on-demand webinar, we discuss why it is important to characterize antibodies based on their physical properties not just by what they bind, and how you can easily do the former via mass spectrometry-based protein sequencing. Key Takeaways Antibodies are generally characterized based on what they bind, not their [...]

Overcoming Irreproducibility in Life Science Research

2021-10-04T16:40:15-04:00September 28th, 2021|Webinars|

Overcoming Irreproducibility in Life Science Research Sponsored by: Rapid Novor, Inc & Absolute Antibody Our team, along with four other industry panelists, discuss ways to safeguard their research through recombinant antibodies, cell culturing procedures, antibody protein sequences, and reference identifiers. Full Webinar Panelists: Andrew Bradbury, CSO at Specifica “Drug-Like Antibodies from [...]

July 2021

How do You Obtain the Sequence of an Antibody?

2021-10-12T13:22:03-04:00July 10th, 2021|Articles|

Antibody sequences are critical for antibody engineering and protein characterization in therapeutic development. For antibody reagent users, knowing the sequences allows them to perform sequence analysis/alignment to identify binding and cross-reactivity so they can conduct rational experiment design.

What is Amino Acid Sequencing?

2021-10-12T13:26:10-04:00July 5th, 2021|Articles|

Amino acid sequencing is the process of identifying the arrangement of amino acids in proteins and peptides. Numerous distinct amino acids have been discovered in nature but all proteins in the human body are comprised of just twenty different types.

June 2021

Major Antibody Databases and Their Applications

2021-10-05T08:24:12-04:00June 18th, 2021|Articles|

Written by María Gerpe, PhD June 18, 2021 Introduction Research publications represent an additional source of validation proof for commercially available antibodies. As such, academic and industry scientists often also rely on publication references to decide which commercial antibody to purchase. Several independent efforts exist to compile such information. For instance, [...]

March 2021

The Underlying Cause of Medical Diagnostic Invalidation

2021-10-14T12:30:50-04:00March 23rd, 2021|Whitepapers|

In vitro diagnostics (IVDs) are one of the most commonly used tools to diagnose conditions and guide treatment decisions, and are often considered the “silent champion” of healthcare. They work by detecting the absence or presence of particular markers or by measuring the concentration of analytes or specific substances.

August 2019

Protein Sequencing & Antibody Species

2021-10-15T09:20:25-04:00August 26th, 2019|Articles|

We are able to sequence any species because we use de novo protein sequencing by tandem mass spectrometry (MS/MS). This type of protein sequencing by mass spectrometry does not rely on existing databases that might introduce bias or wrong amino acid calls.

Protein Sequencing and Protein Purity

2021-10-15T09:25:40-04:00August 12th, 2019|Articles|

How much protein and what purity is needed for protein sequencing? A: We typically accept samples at 100 µg with at least 80% purity, rare in the field. To measure the amount of protein, you can use a microvolume spectrophotometer such as the Nanodrop or a traditional Bradford assay.

Protein Sequencing and Contaminants

2021-10-15T09:28:16-04:00August 5th, 2019|Articles|

The most common protein contaminants include keratin and serum albumin. Other Mass Spectrometry (MS) protein contaminants identified in research labs worldwide by a recent Human Proteome Organization study are casein and E. coli proteins.

February 2017

June 2016

Moving Towards Recombinant Monoclonal Antibodies

2021-10-15T09:36:33-04:00June 30th, 2016|Articles|

Recombinant Monoclonal Antibodies (rAbs) are highly reproducible, customizable and pure alternatives to the traditional antibodies produced by hybridomas. Get the antibody protein sequence, either by DNA sequencing or the de novo protein sequencing technology, you can rest assured that you can have the exact antibody made recombinantly anytime in the future.