Written by María Gerpe, PhD Contents What are recombinant antibodies? What is the difference between recombinant and traditional antibodies? Why are recombinant antibodies important? Types of recombinant antibodies How are recombinant sequences obtained? Additional Resources References What are recombinant antibodies? Recombinant antibodies are [...]
Since 2006, the One Health Initiative (OHI)’s goal has been to demonstrate the inextricable link between humans, animals, and the environment. Certainly, the current global pandemic is a great testament to the ties between climate change, humans, and animals that OHI has been working to highlight. The rise of other zoonotic diseases (e.g., Hendra, and Nipah viruses) not only directly affect humans through disease transmission but may also result in deep impacts to the food supply
Written by Yuning Wang, PhD What is protein purity? When scientists refer to protein purity, they are referring to a sample's properties and homogeneity. Ideally, if a sample contains protein B, the sample should only contain protein B, rather than protein B and x, y, z proteins and non-protein molecules. Furthermore, the homogeneity [...]
Written by Yuning Wang, PhD, and María Gerpe, PhD IgBLAST Definition Developed by the National Center for Biotechnology Information (NCBI), IgBlast is a tool for the analysis of immunoglobulin and T cell receptor sequences in FASTA format. Using Basic Local Alignment Search Tool (BLAST) technology, IgBlast identifies similar sites within nucleotide or [...]
Abstract In this on-demand webinar, we briefly cover the fundamentals of protein sequencing, how researchers have benefited from implementing protein sequencing into their pipelines, and discuss how Rapid Novor is able to routinely and robustly achieve 100% accuracy and 100% coverage for both monoclonal and oligoclonal antibodies. Webinar details [...]
Abstract In this on-demand webinar, we discuss why it is important to characterize antibodies based on their physical properties not just by what they bind, and how you can easily do the former via mass spectrometry-based protein sequencing. Key Takeaways Antibodies are generally characterized based on what they bind, not their [...]
Overcoming Irreproducibility in Life Science Research Sponsored by: Rapid Novor, Inc & Absolute Antibody Our team, along with four other industry panelists, discuss ways to safeguard their research through recombinant antibodies, cell culturing procedures, antibody protein sequences, and reference identifiers. Full Webinar Panelists: Andrew Bradbury, CSO at Specifica “Drug-Like Antibodies from [...]
Antibody sequences are critical for antibody engineering and protein characterization in therapeutic development. For antibody reagent users, knowing the sequences allows them to perform sequence analysis/alignment to identify binding and cross-reactivity so they can conduct rational experiment design.
Amino acid sequencing is the process of identifying the arrangement of amino acids in proteins and peptides. Numerous distinct amino acids have been discovered in nature but all proteins in the human body are comprised of just twenty different types.
Written by María Gerpe, PhD June 18, 2021 Introduction Research publications represent an additional source of validation proof for commercially available antibodies. As such, academic and industry scientists often also rely on publication references to decide which commercial antibody to purchase. Several independent efforts exist to compile such information. For instance, [...]
We are able to sequence any species because we use de novo protein sequencing by tandem mass spectrometry (MS/MS). This type of protein sequencing by mass spectrometry does not rely on existing databases that might introduce bias or wrong amino acid calls.
We recommend that you ship your samples either lyophilized or in solution. If your samples are already lyophilized it is best to ship them to us in that form, they can be shipped at ambient temperatures, and are suitable for transfers longer than 24h.
How much protein and what purity is needed for protein sequencing? A: We typically accept samples at 100 µg with at least 80% purity, rare in the field. To measure the amount of protein, you can use a microvolume spectrophotometer such as the Nanodrop or a traditional Bradford assay.
The most common protein contaminants include keratin and serum albumin. Other Mass Spectrometry (MS) protein contaminants identified in research labs worldwide by a recent Human Proteome Organization study are casein and E. coli proteins.
Nowadays, DNA sequencing is so popular that it is easy to forget that the first sequenced biological material was protein – insulin, by Sanger. Sanger, and another researcher, Edman, separately pioneered protein sequencing.
Recombinant Monoclonal Antibodies (rAbs) are highly reproducible, customizable and pure alternatives to the traditional antibodies produced by hybridomas. Get the antibody protein sequence, either by DNA sequencing or the de novo protein sequencing technology, you can rest assured that you can have the exact antibody made recombinantly anytime in the future.