A Large-Scale Comparison of MS-based Antibody De Novo Protein Sequencing and Targeted DNA Sequencing Introduction The DNA sequences of antibodies are highly diverse due to the V-(D)-J recombination and hypersomatic mutations. As such, a new antibody of interest is unlikely to appear in any existing sequence database. Consequently, the database search approach commonly used in [...]
Learn about what's included in our REmAb™ Service Report, including: notable observations, experiment procedures and sequence confirmations. It's time to remove the guesswork and increase your specificity.
In this video you will learn about the advantages to protein sequencing by mass spectrometry; including the ability to sequence without having access to the original cell line. For more information, check out our resource: De Novo Antibody Protein Sequencing with Mass Spectrometry.
Introduction Monoclonal antibodies (mAbs) are widely used in research, diagnosis, and pharmaceutical purposes. Lately, the relatively lower quality of the research-purpose mAbs is a point of concern within the research community¹,². The problems include the lack of validation and low reproducibility. As most of the validation is done by immunoassay, the extent of protein contamination, [...]