Rapid Novor offers the Sequencing + Expression service to deliver antibodies that works. We directly sequence the antibody protein and then make a recombinant version of it. Our service guarantees that the antibody we produced binds to the same antigen with similar affinity as the antibody sample provided.
The standard Sequencing + Expression service consists of the following steps:
- Sequence the antibody protein using our proprietary antibody protein sequencing service.
- Gene synthesis
- Cloning into pUV vectors
- Transient transfection of HEK293 cells
- Affinity purification
- Quality control to ensure purity (>98%), low endotoxin (<1EU/mg) and no albumin contamination