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Antibody Protein Sequencing 2017-01-19T15:40:10+00:00

Antibody Protein Sequencing

Free Consultation

Key Benefits

  • The ability to reconstruct the hybridoma from the monoclonal antibody protein sample
  • Accurately determine the amino acid sequences in the variable-regions, particularly the CDR regions
  • Look at amino acid level evidences to gauge the sequencing accuracy
  • Works for all antibody isotypes (IgG, IgA, IgM, etc) and species (human, mouse, rat, rabbit, hamster, etc)
  • Comprehensive analyses to confirm your antibody product primary sequence, with optional services to analyze PTMs, glycosylation and sequence variance.

Service Overview

Rapid Novor offers antibody de novo sequencing service. We directly sequence the antibody protein using multiple enzyme digestion and high resolution LC-MS/MS. Our service guarantees full length sequencing of the heavy and light chains.

Our sequencing process does not require hybridoma, mRNA, or other sequence information. It provides a direct and independent insight into your antibody sample.

Accurate – We guarantee at least 5X coverage of CDRs in the variable regions. Every amino acid in the CDRs is confidently supported by fragment ions in at least five MS2 scans. For isomeric Leu and Ile, we strive to make correct inference from enzyme specificity and statistics in antibody database.

Comprehensive – Beyond full sequence coverage, we look at amino acid level evidences to ensure sequencing accuracy. We offer comprehensive characterization of the antibody protein. Our optional services includes the analyses of PTMs, glycosylation, disulphide bridges, contaminants, and other aspects customized to your needs.

Efficient – We use minimal amount of antibody sample (0.2 mg) and deliver results within 15 business days with consistent quality.

Antibody Protein Sequencing - Sequence Coverage View

Method

Our team specializes in protein mass spectrometry and informatics. We develop proprietary workflow and software for antibody de novo sequencing. Our standard procedure consists of the following steps.

  1. Digest the antibody protein into overlapping peptides with multiple enzymes.
  2. Analyze the digestions with LC-MS/MS using a Thermo Orbitrap mass spectrometer.
  3. Characterize overlapping peptides with de novo peptide sequencing.
  4. Automated antibody sequence assembly from characterized peptides.
  5. Result examination and annotation by our scientists.

Antibody Protein Sequencing Experiment Workflow

Variable Region Annotation

Variable Region Annotation

Leu/Ile Inference

Leucine/Isoleucine Inference

Pricing

SampleTimelineDeliverablePrice
Monoclonal antibody
(>0.2 mg; 95% pure)
15 business days after receiving the sample
  • Full sequence of heavy and light chains
  • Full sequence coverage by LC-MS/MS
  • Comprehensive report
  • Raw LC-MS/MS data
Quote
  • Discounts – We offer price discount to new customers and volume orders.
  • Money-back guarantee – We guarantee at least 5X coverage of the CDRs, 3X coverage for 95% of the sequence, and 1X coverage for the full length. If the target coverage is not met, you will be fully refunded.

Contact us and discuss your requirement in detail. We would like to help.

Free Consultation