Rapid Novor offers antibody de novo sequencing service. We directly sequence the antibody protein using multiple enzyme digestion and high resolution LC-MS/MS. Our service guarantees full length sequencing of the heavy and light chains.
Our sequencing process does not require hybridoma, mRNA, or other sequence information. It provides a direct and independent insight into your antibody sample.
Accurate – We guarantee at least 5X coverage of CDRs in the variable regions. Every amino acid in the CDRs is confidently supported by fragment ions in at least five MS2 scans. For isomeric Leu and Ile, we strive to make correct inference from enzyme specificity and statistics in antibody database.
Comprehensive – Beyond full sequence coverage, we look at amino acid level evidences to ensure sequencing accuracy. We offer comprehensive characterization of the antibody protein. Our optional services includes the analyses of PTMs, glycosylation, disulphide bridges, contaminants, and other aspects customized to your needs.
Efficient – We use minimal amount of antibody sample (0.2 mg) and deliver results within 15 business days with consistent quality.